CAJ | 학술논문

建立了离子排斥色谱法（ ion-exclusion chromatography，IEC）测定黄酒中有机酸含量的分析方法。使用Waters 离子排斥色谱柱（300 mm×7.8 mm，7μm），流动相为 H 2 SO 4溶液（ A）与乙腈（ B）的混合溶液（体积比为98：2），线性梯度程序：0~40 min，流动相 A 的浓度由0.01 mol / L 上升到0.02 mol / L；40~50 min，流动相 A 的浓度为0.01 mol / L ；流速为0.5 mL / min，柱温50℃，进样量10μL，检测波长210 nm。结果表明，该方法可在30 min内实现草酸、马来酸、柠檬酸、酒石酸、苹果酸、抗坏血酸、琥珀酸、乳酸、富马酸、乙酸、丙酸、异丁酸和丁酸的完全分离与定量，13种有机酸在0.001~1.000 g / L 范围内线性关系良好，回归方程的线性相关系数在0.9997以上。黄酒中13种有机酸的加标回收率为93.4％~103.8％，相对标准偏差为0.1％~1.5％（n =5）。该方法简单快捷、准确、重复性好，可用于黄酒中有机酸的测定。
건립료리자배척색보법（ ion-exclusion chromatography，IEC）측정황주중유궤산함량적분석방법。사용Waters 리자배척색보주（300 mm×7.8 mm，7μm），류동상위 H 2 SO 4용액（ A）여을정（ B）적혼합용액（체적비위98：2），선성제도정서：0~40 min，류동상 A 적농도유0.01 mol / L 상승도0.02 mol / L；40~50 min，류동상 A 적농도위0.01 mol / L ；류속위0.5 mL / min，주온50℃，진양량10μL，검측파장210 nm。결과표명，해방법가재30 min내실현초산、마래산、저몽산、주석산、평과산、항배혈산、호박산、유산、부마산、을산、병산、이정산화정산적완전분리여정량，13충유궤산재0.001~1.000 g / L 범위내선성관계량호，회귀방정적선성상관계수재0.9997이상。황주중13충유궤산적가표회수솔위93.4％~103.8％，상대표준편차위0.1％~1.5％（n =5）。해방법간단쾌첩、준학、중복성호，가용우황주중유궤산적측정。
An ion-exclusion chromatographic method for the simultaneous determination of organic acids in rice wine was developed. An IC-Pak Ion Exclusion column(300 mm×7. 8 mm, 7 μm)was used at 50 ℃ . The mobile phases were H2SO4(phase A)and acetonitrile(phase B) (98 :2,v/v)at a flow rate of 0. 5 mL / min . The gradient elution program was as follows:0-40 min,0. 01mol/LH2SO4 to 0. 02 mol/LH2SO4;40 - 50 min,0. 01mol/LH2SO4 . The injection volume was 10 μL. The detection wavelength was set at 210 nm. The results showed that oxalic acid,maleic acid,citric acid,tartaric acid,malic acid,ascorbic acid,succinic acid,lactic, fumaric acid,acetic acid,propionic acid,isobutyric acid and butyric acid were completely sep-arated and determined in 30 min. The linear correlation coefficients were above 0. 9997 in the range of 0. 001-1. 000 g / L. Under the optimized conditions,the recoveries of organic acids in rice wine were in the range of 93. 4% -103. 8% with the relative standard deviations(RSDs,n =5)of 0. 1% - 1. 5% . This method is feasible,convenient,fast,accurate and applicable for the quantitative analysis of the organic acids in rice wine.