CAJ | 학술논문

生物表面活性剂鼠李糖脂是微生物在一定条件下产生的次级代谢产物，其分子具有极性亲水基团和非极性亲油基团结构，通常表现出很高的表面活性和界面优先分配能力。可靠的分离提纯方法和成分鉴定手段是鼠李糖脂生产工艺成功的重要保证。实验通过好氧发酵培养铜绿假单胞菌CCTCCAB93066、酸沉降分离得到鼠李糖脂后，利用柱色谱提纯技术得到纯化的鼠李糖脂的单糖脂和二糖脂，最后采用高效液相色谱-质谱联用法进行成分鉴定。结果显示这两种鼠李糖脂均含有3种主要成分，其中单糖脂的主要成分为RhaC10C10、RhaC10C12-H2、RhaC10C12，二糖脂的主要成分为Rha2C10C10、Rha2C10C12-H2、Rha2C10C12。该研究结果表明，铜绿假单胞菌CCTCCAB93066是一种良好的鼠李糖脂产生菌；酸沉降-柱色谱技术可以用于鼠李糖脂的深度提纯，且有较好的效果；而高效液相色谱-质谱联用技术对鼠李糖脂成分鉴定具有灵敏度高和准确性好等优点，是一种较为可靠的检测方法。
생물표면활성제서리당지시미생물재일정조건하산생적차급대사산물，기분자구유겁성친수기단화비겁성친유기단결구，통상표현출흔고적표면활성화계면우선분배능력。가고적분리제순방법화성분감정수단시서리당지생산공예성공적중요보증。실험통과호양발효배양동록가단포균CCTCCAB93066、산침강분리득도서리당지후，이용주색보제순기술득도순화적서리당지적단당지화이당지，최후채용고효액상색보-질보련용법진행성분감정。결과현시저량충서리당지균함유3충주요성분，기중단당지적주요성분위RhaC10C10、RhaC10C12-H2、RhaC10C12，이당지적주요성분위Rha2C10C10、Rha2C10C12-H2、Rha2C10C12。해연구결과표명，동록가단포균CCTCCAB93066시일충량호적서리당지산생균；산침강-주색보기술가이용우서리당지적심도제순，차유교호적효과；이고효액상색보-질보련용기술대서리당지성분감정구유령민도고화준학성호등우점，시일충교위가고적검측방법。
Biosurfactant rhamnolipid is a metabolic intermediate produced by microorganisms under a certain condition. There are the polar hydrophilic group and the non-polar hydrophobic group in rhamnolipid molecule which always exhibits high surface or interfacial activity. A relia-ble separation and purification method as well as component identification technique is essential for success of production process. The rhamnolipid was produced by aerobic fermentation using Pseudomonas aeruginosa CCTCC AB93066 in this study. It was separated from the culture by acid precipitation and purified by column chromatography until two groups of monorhamnolipid and dirhamnolipid were obtained. High performance liquid chromatography with mass spec-trometry(HPLC-MS)examination showed that either the monorhamnolipid or the dirhamnolip-id contained three major species. They were RhaC 10 C 10 ,RhaC 10 C 12-H 2 ,RhaC 10 C 12 for monorh-amnolipid and Rha 2 C 10 C 10 ,Rha 2 C 10 C 12-H 2 ,Rha 2 C 10 C 12 for dirhamnolipid. The results of the study suggested that Pseudomonas aeruginosa CCTCC AB93066 is a good strain for rhamnolipid production. Acid precipitation-column chromatography technique is good for purification of rh-amnolipid. Meanwhile,HPLC-MS is a reliable method for identifying components of rhamnolipid with high sensitivity and accuracy.