CAJ | 학술논문

制备了一种基于螺旋线形跨尺度结构的酶传感器,并对该传感器进行了表征和性能测试.将准30μm键合Au丝以螺旋线方式手工缠绕在准125μm光纤纤芯上,在该Au螺旋线上用水浴法合成ZnO纳米线,得到螺旋线形跨尺度结构；在ZnO纳米线上物理吸附葡萄糖氧化酶( GOD),制备了葡萄糖传感器工作电极.利用扫描电子显微镜( SEM)图像和MatLab图像处理算子分别对螺旋线形跨尺度结构表面形貌及其上活力为50 units/mg的GOD吸附效果进行了定性和定量表征,分析了非高斯粗糙表面与GOD吸附效果的影响关系.基于三电极体系采用循环伏安法和计时安培法测试了制备的12个工作电极的性能,测得该类传感器的灵敏度为(1.410依0.665)μA·L/(mmol·cm2),线性范围为0~(4.292依0.652) mmol/L, Michaelis-Menten常数为(3.571依1.280) mmol/L,检出限为(14.085依8.393)μmol/L.使用活力更高的GOD可以得到性能更好的螺旋线形跨尺度葡萄糖传感器.该类传感器可广泛应用于医药、生物、食品加工及环境监测领域中尿酸、尿素、胆固醇、过氧化氢和苯酚等的检测.
제비료일충기우라선선형과척도결구적매전감기,병대해전감기진행료표정화성능측시.장준30μm건합Au사이라선선방식수공전요재준125μm광섬섬심상,재해Au라선선상용수욕법합성ZnO납미선,득도라선선형과척도결구；재ZnO납미선상물리흡부포도당양화매( GOD),제비료포도당전감기공작전겁.이용소묘전자현미경( SEM)도상화MatLab도상처리산자분별대라선선형과척도결구표면형모급기상활력위50 units/mg적GOD흡부효과진행료정성화정량표정,분석료비고사조조표면여GOD흡부효과적영향관계.기우삼전겁체계채용순배복안법화계시안배법측시료제비적12개공작전겁적성능,측득해류전감기적령민도위(1.410의0.665)μA·L/(mmol·cm2),선성범위위0~(4.292의0.652) mmol/L, Michaelis-Menten상수위(3.571의1.280) mmol/L,검출한위(14.085의8.393)μmol/L.사용활력경고적GOD가이득도성능경호적라선선형과척도포도당전감기.해류전감기가엄범응용우의약、생물、식품가공급배경감측영역중뇨산、뇨소、담고순、과양화경화분분등적검측.
In this paper, a spirally hierarchical structure-based enzymatic glucose sensor was constructed and characterized. An Au fiber of diameter 30 μm was spiraled on an optical fiber core of diameter 125 μm, for-ming a cylindrical spiral. ZnO nanowires were hydrothermally synthesized on the surface of the cylindrical spi-ral, generating a spirally hierarchical structure. Glucose oxidase( GOD) was physically adsorbed on the ZnO nanowires, producing the working electrode of the glucose sensor. Based on micrographs of scanning electron microscopy( SEM) and operators of filtration, line edge detection and fitting of MatLab software, the surface morphology of the spirally hierarchical structure and the adsorption of GOD of activity 50 units/mg on its sur-face were qualitatively and quantitatively characterized, and the effect of the surface morphology of this non-Gaussian rough surface on GOD adsorption also was briefly investigated. Based on the three-electrode system, cyclic voltammetry and amperometric response were employed to evaluate the performance of 12 glucose sen-sors. The sensitivity, the linear range, Michaelis-Menten constant and the detection limit of these 12 glucose sensors were determined to be (1. 410±0. 665) μA·L/(mmol·cm2), 0 -(4. 292±0. 652) mmol/L, (3. 571±1. 280) mmol/L, and (14. 085±8. 393) μmol/L, respectively. With GOD of higher activity used, a glucose sensor with better performance can be constructed. This three-electrode system-based glucose sensor with a spirally hierarchical structure can be employed to detect concentrations of uric acid, urea, cholesterol, hydroperoxide and phenol in medicine, biology, food industry and environmental monitoring domain.