CAJ | 학술논문

目的：探讨在牛Ⅱ型胶原诱导性关节炎模型（ Collagen-induced arthritis ，CIA）小鼠的诱导过程中，弗氏完全佐剂（ FCA）中的卡介苗（ BCG）浓度和CIA小鼠关节炎严重程度的关系。方法：将牛Ⅱ型胶原蛋白溶液与含不同BCG的FCA等体积混合乳化，在小鼠尾根部注射进行免疫。免疫后观察小鼠的相关指标包括体重、发病率、血清TNF-α、关节炎指数及小鼠关节和脾脏的病理变化。结果：与1 mg/ml卡介苗组相比，4 mg/ml卡介苗组诱导的CIA小鼠发病早，关节炎指数、关节和脾脏病理评分升高及小鼠体重下降更明显，血清TGF-α明显升高，差异有统计学意义。结论：在进行CIA小鼠诱导时，BCG浓度和CIA小鼠关节炎严重程度呈正相关，实验中可以根据对动物的要求，选择含不同BCG的佐剂来诱导CIA小鼠。
목적：탐토재우Ⅱ형효원유도성관절염모형（ Collagen-induced arthritis ，CIA）소서적유도과정중，불씨완전좌제（ FCA）중적잡개묘（ BCG）농도화CIA소서관절염엄중정도적관계。방법：장우Ⅱ형효원단백용액여함불동BCG적FCA등체적혼합유화，재소서미근부주사진행면역。면역후관찰소서적상관지표포괄체중、발병솔、혈청TNF-α、관절염지수급소서관절화비장적병리변화。결과：여1 mg/ml잡개묘조상비，4 mg/ml잡개묘조유도적CIA소서발병조，관절염지수、관절화비장병리평분승고급소서체중하강경명현，혈청TGF-α명현승고，차이유통계학의의。결론：재진행CIA소서유도시，BCG농도화CIA소서관절염엄중정도정정상관，실험중가이근거대동물적요구，선택함불동BCG적좌제래유도CIA소서。
Objective:To determine the correlation of BCG concentration in Freund complete adjuvant ( FCA) and severity of arthritis during arthritis establishment in DBA 1/j male mice induced by bovine type Ⅱcollagen.Methods:CIA was induced by the im-munization of DBA1/j mice with bovine type Ⅱcollagen and BCG of various concentrations dissolved in FCA.To ascertain the effects administering the collagen booster,CIA-related features(including body weight,clinical scoring of arthritis),TNF-αin serum and the histopathological changes in the spleen and the joints regions were measured.Results:4 mg/ml BCG induced more serious arthritis than 1 mg/ml in DBA1/j mice after collagen exposure.Conclusion:There is a positive correlation of arthritis severity with BCG.Which indicates that selection of adjuvant with suitable BCG concentration could determine pathological outcome in CIA mouse model .