结核分枝杆菌PhoPR双组分系统与不同毒力结核分枝杆菌致病性的相关性研究
결핵분지간균PhoPR쌍조분계통여불동독력결핵분지간균치병성적상관성연구
Correlation study between Mycobacterium tuberculosis PhoPR two-component system and pathogenicity of different virulent Mycobacterium tuberculosis
  • 万方数据
    中国免疫学杂志 중국면역학잡지
    CHINESE JOURNAL OF IMMUNOLOGY
    2014年 10期 1320-1324,1329 ,共6页

    邬博%吴芳%章乐%吴江东%张辉%张春军%曹旭东%朱彬%李瑞山%何丽%王钊%张玉清%庄睿%李文娟%梁晨%樊超%张万江

    오박%오방%장악%오강동%장휘%장춘군%조욱동%주빈%리서산%하려%왕쇠%장옥청%장예%리문연%량신%번초%장만강

    结核分枝杆菌%致病性%PhoPR双组分系统 結覈分枝桿菌%緻病性%PhoPR雙組分繫統
    결핵분지간균%치병성%PhoPR쌍조분계통
    Mycobacterium tuberculosis%Pathogenicity%PhoPR two-component system
    目的：通过比较分析卡介苗株（BCG）、结核分枝杆菌国际标准无毒株（H37Ra）、结核分枝杆菌国际标准强毒株（ H37Rv）、新疆地区流行的优势强毒株结核分枝杆菌临床分离株（ XJ-MTB）的PhoP基因和PhoR基因的表达水平差异，探讨研究结核分枝杆菌PhoPR双组分系统与不同毒力结核分枝杆菌的致病性是否具有相关性。方法：首先提取上述四种不同毒力结核分枝杆菌菌株的总RNA，并进行完整性鉴定；再运用SYBR Green I实时荧光定量PCR技术检测各组菌株中PhoP基因和PhoR基因的表达水平；最后比较不同毒力结核分枝杆菌PhoP基因和PhoR基因的表达水平差异。结果：四种不同毒力菌株PhoP基因的相对表达量，由高到低依次为XJ-MTB（9.05）、H37Rv（1.00）、H37Ra（0.25）、BCG（0.08），且四种菌株中PhoP基因的表达有显著的统计学差异（P＜0.05）；同时四种不同毒力菌株PhoR基因的相对表达量，由高到低依次为XJ-MTB（5.72）、H37Rv（1.00）、H37Ra（0.18）、BCG（0.07），且四种菌株中PhoR基因的表达有显著的统计学差异（P＜0.05）。其中XJ-MTB菌株PhoP基因和PhoR基因的表达水平与BCG、H37Rv、H37Ra相比存在显著的统计学差异（P＜0.05）；H37Rv菌株PhoP基因和PhoR基因的表达与BCG、H37Ra相比存在显著的统计学差异（P＜0.05）；而BCG菌株PhoP基因和PhoR基因的表达与H37Ra相比统计学差异不显著（ P＞0.05）。结论：结核分枝杆菌PhoPR双组分系统中PhoP基因和PhoR基因在不同毒力结核分枝杆菌中的表达存在差异，并且该系统与不同毒力结核分枝杆菌的致病性存在相关性。
    목적：통과비교분석잡개묘주（BCG）、결핵분지간균국제표준무독주（H37Ra）、결핵분지간균국제표준강독주（ H37Rv）、신강지구류행적우세강독주결핵분지간균림상분리주（ XJ-MTB）적PhoP기인화PhoR기인적표체수평차이，탐토연구결핵분지간균PhoPR쌍조분계통여불동독력결핵분지간균적치병성시부구유상관성。방법：수선제취상술사충불동독력결핵분지간균균주적총RNA，병진행완정성감정；재운용SYBR Green I실시형광정량PCR기술검측각조균주중PhoP기인화PhoR기인적표체수평；최후비교불동독력결핵분지간균PhoP기인화PhoR기인적표체수평차이。결과：사충불동독력균주PhoP기인적상대표체량，유고도저의차위XJ-MTB（9.05）、H37Rv（1.00）、H37Ra（0.25）、BCG（0.08），차사충균주중PhoP기인적표체유현저적통계학차이（P＜0.05）；동시사충불동독력균주PhoR기인적상대표체량，유고도저의차위XJ-MTB（5.72）、H37Rv（1.00）、H37Ra（0.18）、BCG（0.07），차사충균주중PhoR기인적표체유현저적통계학차이（P＜0.05）。기중XJ-MTB균주PhoP기인화PhoR기인적표체수평여BCG、H37Rv、H37Ra상비존재현저적통계학차이（P＜0.05）；H37Rv균주PhoP기인화PhoR기인적표체여BCG、H37Ra상비존재현저적통계학차이（P＜0.05）；이BCG균주PhoP기인화PhoR기인적표체여H37Ra상비통계학차이불현저（ P＞0.05）。결론：결핵분지간균PhoPR쌍조분계통중PhoP기인화PhoR기인재불동독력결핵분지간균중적표체존재차이，병차해계통여불동독력결핵분지간균적치병성존재상관성。
    Objective:To explore the correlation between Mycobacterium tuberculosis PhoPR two-component system and the pathogenicity of different virulent MTB by analysing the expression levels difference of PhoP gene and PhoR gene in BCG ,H37Ra, H37Rv and XJ-MTB respectively.Methods:Total RNA extracted from four different virulent MTB strains and the integrity of total RNA were identified by using agarose gel electrophoresis.The expression of PhoP gene and PhoR gene were quantified by using SYBR Green I FQ-PCR.The expression levels difference of these genes were compared in different virulent MTB strains .Results: The relative expression levels of PhoP gene in between four different virulent MTB strains from high to low were XJ -MTB(9.05),H37Rv(1.00), H37Ra(0.25),BCG(0.08) respectively ,and the expression levels difference of PhoP gene were statistically significant in different virulent MTB strains ( P<0.05 );the relative expression levels of PhoR gene in four different virulent MTB strains from high to low were XJ-MTB(5.72),H37Rv(1.00),H37Ra(0.18),BCG(0.07) respectively,and the expression levels difference of PhoR gene were sta-tistically significant in different virulent MTB strains ( P<0.05 ).The expression levels of PhoP gene and PhoR gene at XJ-MTB were statistically significant difference compared with BCG ,H37Rv,H37Ra respectively (P<0.05);the expression levels of PhoP gene and PhoR gene at H37Rv were statistically significant difference compared with BCG ,H37Ra respectively (P<0.05);the expression levels&nbsp;of PhoP and PhoR gene at BCG were not statistically significant difference compared with H 37Ra (P>0.05).Conclusion:Significant expression levels difference of PhoP gene and PhoR gene are confirmed in different virulent MTB strains .Therefore,the Mycobacterium tuberculosis PhoPR two-component system is correlated with the pathogenicity in different virulent MTB strains.
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