中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2014年
10期
1309-1312,1319
,共5页
钱江%韩佳%陈鹏%朱春丽%傅仲学
錢江%韓佳%陳鵬%硃春麗%傅仲學
전강%한가%진붕%주춘려%부중학
RNA干扰%Slug%裸鼠%HCT116
RNA榦擾%Slug%裸鼠%HCT116
RNA간우%Slug%라서%HCT116
RNA interference%Slug%Nude mice%HCT116
目的:探讨通过靶向RNA干扰(RNA interference,RNAi)抑制Slug基因的表达,观测对荷瘤裸鼠结直肠癌生长及转移的影响。方法:利用结肠癌细胞株HCT116对24只5周龄裸鼠皮下种植,建立结肠癌裸鼠皮下移植瘤模型,设立空白对照组、阴性对照组及实验组三组,每组8只。分别注射生理盐水、阴性对照质粒及慢病毒载体,观察肿瘤生长情况,绘制肿瘤生长曲线,观察各组间肿瘤生长及淋巴结转移的变化,应用免疫组化、qRT-PCR和Western blot检测Slug基因和蛋白表达情况。结果:Slug基因shRNA实验组与空白对照组和阴性对照组相比,瘤体生长减缓,移植瘤质量明显减小(3.08±0.31 vs 7.37±1.18,7.46±1.16,P<0.01),Slug蛋白表达明显降低(P<0.05);实验组淋巴结阳性率为36.3%(4/11),与阴性对照组77.8%(14/18)及空白对照组68.4%(13/19)相比较(P<0.01)。结论:靶向Slug的RNA干扰可以显著抑制结肠癌裸鼠模型的生长、淋巴结转移以及癌组织中Slug基因蛋白的表达,可能成为结肠癌基因治疗的潜在分子靶点。
目的:探討通過靶嚮RNA榦擾(RNA interference,RNAi)抑製Slug基因的錶達,觀測對荷瘤裸鼠結直腸癌生長及轉移的影響。方法:利用結腸癌細胞株HCT116對24隻5週齡裸鼠皮下種植,建立結腸癌裸鼠皮下移植瘤模型,設立空白對照組、陰性對照組及實驗組三組,每組8隻。分彆註射生理鹽水、陰性對照質粒及慢病毒載體,觀察腫瘤生長情況,繪製腫瘤生長麯線,觀察各組間腫瘤生長及淋巴結轉移的變化,應用免疫組化、qRT-PCR和Western blot檢測Slug基因和蛋白錶達情況。結果:Slug基因shRNA實驗組與空白對照組和陰性對照組相比,瘤體生長減緩,移植瘤質量明顯減小(3.08±0.31 vs 7.37±1.18,7.46±1.16,P<0.01),Slug蛋白錶達明顯降低(P<0.05);實驗組淋巴結暘性率為36.3%(4/11),與陰性對照組77.8%(14/18)及空白對照組68.4%(13/19)相比較(P<0.01)。結論:靶嚮Slug的RNA榦擾可以顯著抑製結腸癌裸鼠模型的生長、淋巴結轉移以及癌組織中Slug基因蛋白的錶達,可能成為結腸癌基因治療的潛在分子靶點。
목적:탐토통과파향RNA간우(RNA interference,RNAi)억제Slug기인적표체,관측대하류라서결직장암생장급전이적영향。방법:이용결장암세포주HCT116대24지5주령라서피하충식,건립결장암라서피하이식류모형,설립공백대조조、음성대조조급실험조삼조,매조8지。분별주사생리염수、음성대조질립급만병독재체,관찰종류생장정황,회제종류생장곡선,관찰각조간종류생장급림파결전이적변화,응용면역조화、qRT-PCR화Western blot검측Slug기인화단백표체정황。결과:Slug기인shRNA실험조여공백대조조화음성대조조상비,류체생장감완,이식류질량명현감소(3.08±0.31 vs 7.37±1.18,7.46±1.16,P<0.01),Slug단백표체명현강저(P<0.05);실험조림파결양성솔위36.3%(4/11),여음성대조조77.8%(14/18)급공백대조조68.4%(13/19)상비교(P<0.01)。결론:파향Slug적RNA간우가이현저억제결장암라서모형적생장、림파결전이이급암조직중Slug기인단백적표체,가능성위결장암기인치료적잠재분자파점。
Objective:To explore the influence of RNAi mediated Slug silencing on the growth and metastasis of colon cancer in nude mice.Methods: HCT116 colon cancer cells use for 24 five-week-old nude mice implanted subcutaneously , established colon cancer xenograft model in nude mice ,all divided into blank control group ,negative control group and the experimental group ,each group had eight nude mices.All group were injected with saline , negative plasmid and lentivirus vectors respectively.Tumor growth was observed and draw tumor curved growth ,changes in tumor growth and lymph node metastasis between the groups were observed ,Slug gene and protein expression were detected by immunohistochemistry ,qRT-PCR and Western blot analysis.Results: Slug gene shRNA intervention group compared with the control group and negative control group ,tumor grew slower ,tumor mass was significantly reduced (3.08±0.31 vs 7.37±1.18,7.46±1.16,P<0.01),experimental group of lymph node-positive rate was 36.3%( 4 /11 ) ,compared to the negative control group 77.8% ( 14/18 ) and the control group was 68.4% ( 13/19 ) ( P<0.01 ).Conclusion: Targeted Slug RNA interference can significantly inhibit the growth of colon cancer in nude mice ,lymph node metastasis and the expression of the gene protein in cancer tissue ,Slug may be a potential molecular target for colon cancer gene therapy.