云南中医学院学报
雲南中醫學院學報
운남중의학원학보
JOURNAL OF YUNNAN COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
1期
12-14,25
,共4页
防感煎剂%甲1型流感病毒%TNF-α%IL-6
防感煎劑%甲1型流感病毒%TNF-α%IL-6
방감전제%갑1형류감병독%TNF-α%IL-6
Fanggan decoction%influenza a virus(H1N1)%TNF-α%IL-6
目的：探讨防感煎剂对H1N1流感病毒感染小鼠TNF-α、IL-6的影响，进一步了解防感煎剂的疗效机理。方法选用清洁级ICR雄性小鼠96只，随机分为4组：正常对照组、模型对照组、防感煎剂组、达菲对照组。实验组予防感煎剂灌胃给药，达菲对照组予达菲灌胃给药，正常对照组和模型对照组则用生理盐水灌胃，在灌胃给药第3天开始用H1N1流感病毒液滴鼻造模，造模后的第1、5、9、13天每组随机处死6只小鼠，收集肺组织标本，用于H1N1病毒核酸提取，收集血清，用ELISA法检测肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）含量。结果1、防感煎剂组小鼠一般状况优于模型组。2、防感煎剂组小鼠肺组织H1N1病毒含量低于模型组（P<0.05）。3、防感煎剂组小鼠血清TNF-α、IL-6水平低于模型组（P<0.01）。结论防感煎剂可抑制H1NI病毒复制，抑制细胞因子TNF-α、IL-6的表达，从而减轻由炎性介质过度释放引起的免疫病理损伤，由此推断防感煎剂可能通过调节免疫功能来控制感染、改善症状。
目的：探討防感煎劑對H1N1流感病毒感染小鼠TNF-α、IL-6的影響，進一步瞭解防感煎劑的療效機理。方法選用清潔級ICR雄性小鼠96隻，隨機分為4組：正常對照組、模型對照組、防感煎劑組、達菲對照組。實驗組予防感煎劑灌胃給藥，達菲對照組予達菲灌胃給藥，正常對照組和模型對照組則用生理鹽水灌胃，在灌胃給藥第3天開始用H1N1流感病毒液滴鼻造模，造模後的第1、5、9、13天每組隨機處死6隻小鼠，收集肺組織標本，用于H1N1病毒覈痠提取，收集血清，用ELISA法檢測腫瘤壞死因子α（TNF-α）、白細胞介素6（IL-6）含量。結果1、防感煎劑組小鼠一般狀況優于模型組。2、防感煎劑組小鼠肺組織H1N1病毒含量低于模型組（P<0.05）。3、防感煎劑組小鼠血清TNF-α、IL-6水平低于模型組（P<0.01）。結論防感煎劑可抑製H1NI病毒複製，抑製細胞因子TNF-α、IL-6的錶達，從而減輕由炎性介質過度釋放引起的免疫病理損傷，由此推斷防感煎劑可能通過調節免疫功能來控製感染、改善癥狀。
목적：탐토방감전제대H1N1류감병독감염소서TNF-α、IL-6적영향，진일보료해방감전제적료효궤리。방법선용청길급ICR웅성소서96지，수궤분위4조：정상대조조、모형대조조、방감전제조、체비대조조。실험조여방감전제관위급약，체비대조조여체비관위급약，정상대조조화모형대조조칙용생리염수관위，재관위급약제3천개시용H1N1류감병독액적비조모，조모후적제1、5、9、13천매조수궤처사6지소서，수집폐조직표본，용우H1N1병독핵산제취，수집혈청，용ELISA법검측종류배사인자α（TNF-α）、백세포개소6（IL-6）함량。결과1、방감전제조소서일반상황우우모형조。2、방감전제조소서폐조직H1N1병독함량저우모형조（P<0.05）。3、방감전제조소서혈청TNF-α、IL-6수평저우모형조（P<0.01）。결론방감전제가억제H1NI병독복제，억제세포인자TNF-α、IL-6적표체，종이감경유염성개질과도석방인기적면역병리손상，유차추단방감전제가능통과조절면역공능래공제감염、개선증상。
Objective To Explore the influence of Fanggan decoction on TNF alpha and IL-6 in the mice infected by influenza a virus (H1N1),to learn more about Fanggan decoction curative effect mechanism. Methods Choose clean level 96 male ICR mice,were randomly divided into 4 groups:normal control group,model control group,Chinese medicine in Chinese medicine dosage group and positive control group. Sense of Chinese traditional medicine group to prevent decoction medium dose to fill the stomach,positive control group to tamiflu lavage,normal control group and model control group with saline lavage. In for 3 days with the flu virus droplets nasal H1N1 virus infection of animal model is set up. In building 1,5,9,13 days after the random death 6 mice in each group,the serum and lung tissue collection,TNF alpha and IL-6,such as secretion and function. Results 1,Fanggan decoction group is better than the model group in general condition of mice. 2,Fanggan Decoction group had lower H1N1 virus content in lung tissue of mice than those in the model group (P<0. 05). 3,drug intervention group mice blood serum tumor necrosis factor (TNF alpha),IL -6,level significantly decreased,compared with the model group had significant difference (P<0. 01). Conclusion Fanggan decoction can depress the replication of the H1NI virus,depress expression of cytokines TNF-α,IL-6,thereby reducing the excessive release of inflammatory mediators by immune pathological injury caused by inflammation,thus Fanggan decoction can regulate the immune function and improve symptoms.
