CAJ | 학술논문

目的：建立流式细胞术（fcM）分析骨髓巨核细胞 dna 倍体的方法，为小巨核细胞的检测提供更可靠的实验依据。方法对42例 Mds 患者进行骨髓巨核细胞绝对计数并在显微镜下对巨核细胞阶段进行分类；采用 Percoll 密度梯度分离液分离富集骨髓巨核细胞，用异硫氰酸荧光素标记的单克隆抗体标记巨核细胞糖蛋白 iib/iiia（cd41a），用 Pi 标记巨核细胞 dna，利用流式细胞仪分析巨核细胞倍体并进行统计学分析。结果建立了基于 cd41a 和 Pi 双标记的流式细胞术鉴别分析小巨核细胞的方法。结论 cd41a 和 Pi 双标记的流式细胞术检测小巨核细胞的方法比瑞氏-姬姆萨染色方法检出率高。
목적：건립류식세포술（fcM）분석골수거핵세포 dna 배체적방법，위소거핵세포적검측제공경가고적실험의거。방법대42례 Mds 환자진행골수거핵세포절대계수병재현미경하대거핵세포계단진행분류；채용 Percoll 밀도제도분리액분리부집골수거핵세포，용이류청산형광소표기적단극륭항체표기거핵세포당단백 iib/iiia（cd41a），용 Pi 표기거핵세포 dna，이용류식세포의분석거핵세포배체병진행통계학분석。결과건립료기우 cd41a 화 Pi 쌍표기적류식세포술감별분석소거핵세포적방법。결론 cd41a 화 Pi 쌍표기적류식세포술검측소거핵세포적방법비서씨-희모살염색방법검출솔고。
Objective The study analysed the DNA ploid of the bone marrow megakaryocytes by flow cytometry, which provide the dependable experiment foundation for measuring micromegaka- ryocyte.Methods 1. in 42 cases of Mds patients, their bone marrow megakaryocytes were counted and classified by cells differentiation stage.2. the bone marrow megakaryocytes were obtained by Percoll density gradient separation medium. the megakaryocyte glycoprotein iib / iiia (cd41a) were marked with fluorescein isothiocyanate through its corresponding monoclonal antibody, and cells DNA were marked with Pi. then the megakaryocyte ploidy was analyzed by flow cytometry (fcM).results: the method for micromegakaryocytes identification and anlysis was established,which was based on cd41a and Pi double-labeled fcM analysis.Conclusions the micromegakaryocytes detection rate of the flow cytometry with CD41a and PI double marker is higher than that in the Wright-Giemsa staining.