包装与食品机械
包裝與食品機械
포장여식품궤계
PACKAGING AND FOOD MACHINERY
2014年
1期
15-18
,共4页
羊肚菌%深层培养%工艺%菌丝生物量
羊肚菌%深層培養%工藝%菌絲生物量
양두균%심층배양%공예%균사생물량
Morehella esculenta%submerged culture%technology%mycelium biomass
为了探讨羊肚菌的液体发酵工艺，优化液体深层发酵培养基的配方。在单因素试验的基础，进行了多因素正交试验。结果表明，羊肚菌的液体深层发酵培养基最佳配方为：葡萄糖1．5％、玉米粉1．5％、蛋白胨0．3％、黄豆粉0．3％、KH2 PO40．3％、MgSO40．2％、CaCO30．1％、VB10．001％，菌丝生物量达3．10 g·100mL-1；液体培养条件为pH值6．5左右、温度25℃。
為瞭探討羊肚菌的液體髮酵工藝，優化液體深層髮酵培養基的配方。在單因素試驗的基礎，進行瞭多因素正交試驗。結果錶明，羊肚菌的液體深層髮酵培養基最佳配方為：葡萄糖1．5％、玉米粉1．5％、蛋白胨0．3％、黃豆粉0．3％、KH2 PO40．3％、MgSO40．2％、CaCO30．1％、VB10．001％，菌絲生物量達3．10 g·100mL-1；液體培養條件為pH值6．5左右、溫度25℃。
위료탐토양두균적액체발효공예，우화액체심층발효배양기적배방。재단인소시험적기출，진행료다인소정교시험。결과표명，양두균적액체심층발효배양기최가배방위：포도당1．5％、옥미분1．5％、단백동0．3％、황두분0．3％、KH2 PO40．3％、MgSO40．2％、CaCO30．1％、VB10．001％，균사생물량체3．10 g·100mL-1；액체배양조건위pH치6．5좌우、온도25℃。
In order to discuss technology of submerged fermentation of Morchella,the submerged culture medium formula was optimized.Based on single factor experiment,the orthogonal test was made.It is shown that the best formula were glucose 1 .5%,corn powder 1 .5%,peptone 0.3%,soybean powder 0.3%,KH2 PO4 0.3%,MgSO4 0.2%,CaCO3 0.1%,VB1 0.001%,the mycelium biomass could reach 3.1 0 g·1 00mL-1 .The submerged culture conditions were temperature 25℃ and pH 6.5 for Morehella esculenta.
