南京林业大学学报(自然科学版)
南京林業大學學報(自然科學版)
남경임업대학학보(자연과학판)
JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2014年
2期
165-168
,共4页
吕秀立%魏翔莺%尹丽娟%黄芳%张春英
呂秀立%魏翔鶯%尹麗娟%黃芳%張春英
려수립%위상앵%윤려연%황방%장춘영
大叶常绿杜鹃%离体培养%不定芽再生系统
大葉常綠杜鵑%離體培養%不定芽再生繫統
대협상록두견%리체배양%불정아재생계통
broad-leaf evergreen rhododendron%in vitro culture%regeneration system of adventitious buds
以大叶常绿杜鹃无菌苗的嫩叶为试验起始材料,研究了其离体培养和不定芽再生的过程。结果表明:培养基为MS+ZT(1.0 mg/L)+ NAA(0.1 mg/L)时,大叶常绿杜鹃叶片可直接诱导再生不定芽,诱导率达70%;也可以通过愈伤组织途径间接诱导出不定芽,愈伤组织诱导培养基为MS+2,4-D(2.0 mg/L)+KT(0.2 mg/L),增殖最佳培养基为MS+ZT(0.5 mg/L)+IBA(0.1 mg/L),愈伤组织分化的最佳培养基为MS+ZT(0.04 mg/L)+NAA(0.01 mg/L);分化率最高为70%,分化苗数为6~10株;生根培养基为MS+NAA(0.2 mg/L)+IBA(0.4 mg/L)时,生根率可达90%。腐殖土、黄沙、珍珠岩配比为4∶1∶1的混合基质较适合常绿杜鹃试管苗的炼苗移栽,成活率为90%。
以大葉常綠杜鵑無菌苗的嫩葉為試驗起始材料,研究瞭其離體培養和不定芽再生的過程。結果錶明:培養基為MS+ZT(1.0 mg/L)+ NAA(0.1 mg/L)時,大葉常綠杜鵑葉片可直接誘導再生不定芽,誘導率達70%;也可以通過愈傷組織途徑間接誘導齣不定芽,愈傷組織誘導培養基為MS+2,4-D(2.0 mg/L)+KT(0.2 mg/L),增殖最佳培養基為MS+ZT(0.5 mg/L)+IBA(0.1 mg/L),愈傷組織分化的最佳培養基為MS+ZT(0.04 mg/L)+NAA(0.01 mg/L);分化率最高為70%,分化苗數為6~10株;生根培養基為MS+NAA(0.2 mg/L)+IBA(0.4 mg/L)時,生根率可達90%。腐殖土、黃沙、珍珠巖配比為4∶1∶1的混閤基質較適閤常綠杜鵑試管苗的煉苗移栽,成活率為90%。
이대협상록두견무균묘적눈협위시험기시재료,연구료기리체배양화불정아재생적과정。결과표명:배양기위MS+ZT(1.0 mg/L)+ NAA(0.1 mg/L)시,대협상록두견협편가직접유도재생불정아,유도솔체70%;야가이통과유상조직도경간접유도출불정아,유상조직유도배양기위MS+2,4-D(2.0 mg/L)+KT(0.2 mg/L),증식최가배양기위MS+ZT(0.5 mg/L)+IBA(0.1 mg/L),유상조직분화적최가배양기위MS+ZT(0.04 mg/L)+NAA(0.01 mg/L);분화솔최고위70%,분화묘수위6~10주;생근배양기위MS+NAA(0.2 mg/L)+IBA(0.4 mg/L)시,생근솔가체90%。부식토、황사、진주암배비위4∶1∶1적혼합기질교괄합상록두견시관묘적련묘이재,성활솔위90%。
Using the young leaves of aseptic broad leaf evergreen rhododendron seedling as the starting materials for this experiment, the culture conditions in vitro and regeneration of adventitious budswere studied. The results showed that:the best medium composition inducing adventitious buds directly from leaf explants were MS+ZT 1.0 mg/L+NAA 0.1 mg/L, which made the induction rate reach to 70%. The adventitious buds could also be induced from the callus indirectly, the best medium composition for which was MS+2,4-D 2.0 mg/L+KT 0.2 mg/L, the best multiplication medium for cal-lus was MS+ZT 0.5 mg/L+ IBA 0.1mg/L,the best medium for callus differentiation was MS+ZT 0.04 mg/L+NAA 0.01 mg/L, which made differentiation rate reach to 70% and the differentiation number of seedlings 6-10. The rate of rooting could reach to 90% by the medium of MS+NAA 0.2 mg/L +IBA 0.4 mg/L. The mixed compost contained four parts of humus soil, and one part of yellow sand, one part of perlite, which was quite suitable for evergreen rhododendron seed-lings transplanting in vitro, and the survival rate was 90%.