CAJ | 학술논문

以青钱柳成熟种子培养无菌苗，再用无菌苗叶和茎段为外植体进行组织培养，观察了愈伤组织形成及再分化形成不定芽的过程，分析了不同培养基和生长调节物质对青钱柳愈伤组织及不定芽发生的影响。结果表明：（1）MS、WPM和B5培养基均能诱导出愈伤组织，茎段和叶片的诱导率最高分别达到90?00％和86?70％；（2）激素及其浓度对愈伤组织诱导均有显著影响。当加入TDZ质量浓度为0．5 mg/L时，叶愈伤组织诱导率达95?00％；而加入TDZ质量浓度为0．1 mg/L时，茎段诱导率达92．85％；（3）叶愈伤组织在WPM＋1．0 mg/L 6-BA＋0．1 mg/L IBA的培养基上，不定芽的诱导频率最高，达53．85％，这实现了青钱柳由愈伤组织分化形成不定芽和萌发增殖的过程，但萌发率较低。
이청전류성숙충자배양무균묘，재용무균묘협화경단위외식체진행조직배양，관찰료유상조직형성급재분화형성불정아적과정，분석료불동배양기화생장조절물질대청전류유상조직급불정아발생적영향。결과표명：（1）MS、WPM화B5배양기균능유도출유상조직，경단화협편적유도솔최고분별체도90?00％화86?70％；（2）격소급기농도대유상조직유도균유현저영향。당가입TDZ질량농도위0．5 mg/L시，협유상조직유도솔체95?00％；이가입TDZ질량농도위0．1 mg/L시，경단유도솔체92．85％；（3）협유상조직재WPM＋1．0 mg/L 6-BA＋0．1 mg/L IBA적배양기상，불정아적유도빈솔최고，체53．85％，저실현료청전류유유상조직분화형성불정아화맹발증식적과정，단맹발솔교저。
Based on the seedlings cultured with matured seeds of Cyclocarya paliurus in vitro, the induction of adventi-tious shoot from callus of C. paliurus and the effects of basic media and different growth regulators on induction of callus and adventitious shoots were studied with explants of leaves and stem segments of the young seedlings. The results showed that callus of leaves and stem segments of the seedlings could be induced on each basic medium applied in this paper and the highest induction frequency of callus reached 90.00% for stem segment and 86.70% for leaf, respectively. The types and concentrations of hormones had significant effects on callus induction. When 0.5 mg/L TDZ was added, the induction frequency of leaf callus reached 95.00%, while 92.85% induction frequency was achieved for stem segment callus at 0.5 mg/L TDZ added. The greatest induction frequency of adventitious shoots from leaf callus was observed in the medium of WPM+1.0 mg/L 6-BA+0.1 mg/L IBA, which reached to 53.85%. Our results successfully achieved the induction and germination of adventitious shoots from the leaf callus, but the germination rate was still low and more studies should be done in the future.