CAJ | 학술논문

控制和决定微生物细胞各种表型与性状的本质十分复杂，涉及遍布细胞基因组的众多基因或相关基因产物间的动态作用。长期以来，微生物生产菌株大多通过自然选育或人工诱变逐代积累有益突变而来。基因组改组技术是在DNA分子（基因组）水平上模拟生物有性杂交过程，对微生物的全基因组进行随机组合与改良。它是基于突变和重组基础上的育种新技术，建立在传统诱变育种基础上，结合多细胞融合技术，对诱变后的突变库进行多亲本基因组重组，从而使具有不同遗传位点有利突变的各亲本菌株将其优势基因汇集在一起，达到快速进化目标性状。
공제화결정미생물세포각충표형여성상적본질십분복잡，섭급편포세포기인조적음다기인혹상관기인산물간적동태작용。장기이래，미생물생산균주대다통과자연선육혹인공유변축대적루유익돌변이래。기인조개조기술시재DNA분자（기인조）수평상모의생물유성잡교과정，대미생물적전기인조진행수궤조합여개량。타시기우돌변화중조기출상적육충신기술，건립재전통유변육충기출상，결합다세포융합기술，대유변후적돌변고진행다친본기인조중조，종이사구유불동유전위점유리돌변적각친본균주장기우세기인회집재일기，체도쾌속진화목표성상。
Phenotypes and traits of the microbial cells are complex in nature, which involve many genes around the whole genome, as well as their products. Classic breeding, which improves target traits through cycles of mutation and selection, accumulates beneficial mutations in an inefficient way. Crossbreeding techniques based on hybridizing parents of higher animals and plants have successfully met the diverse needs of the species. By reason of many valuable microbes have no sexual hybridization ability, and genome shuffling technology is in the simulation of sexual hybridization process on the DNA molecular level and random combination and improvement in the whole-genome. It is a novel breeding technology based on the basis of the traditional mutation breeding and combined with protoplast fusion technology: firstly, introduced of a mutation procedure as classical breeding technique to produce a mutation library composed of different mutants; then, shuffled and rearranged the mutants genome to accumulate useful diversity by muti-parents protoplast fusion of these mutants to rapidly evolve the target traits.