CAJ | 학술논문

通过对一株具有广谱抗病毒活性的放线菌菌株Yn168的形态、培养特征、生理生化特征及16 S rDNA序列分析，对该放线菌进行了菌种鉴定，并研究了不同发酵条件对该菌发酵液抗病毒活性的影响。鉴定结果表明，该菌株为一株小链霉菌（ Streptomyces parvus）。不同发酵条件下发酵液抗病毒活性检测表明，当种子培养24 h、种子液接种量为8％、培养温度为28℃、初始pH值为7．0、摇床转速为200 r/min时，发酵液对烟草花叶病毒（ Tobacco mosaic virus ， TMV）的抑制活性最强，枯斑抑制率在86％以上。
통과대일주구유엄보항병독활성적방선균균주Yn168적형태、배양특정、생리생화특정급16 S rDNA서렬분석，대해방선균진행료균충감정，병연구료불동발효조건대해균발효액항병독활성적영향。감정결과표명，해균주위일주소련매균（ Streptomyces parvus）。불동발효조건하발효액항병독활성검측표명，당충자배양24 h、충자액접충량위8％、배양온도위28℃、초시pH치위7．0、요상전속위200 r/min시，발효액대연초화협병독（ Tobacco mosaic virus ， TMV）적억제활성최강，고반억제솔재86％이상。
A actinomycete strain Yn168 with broad-spectrum antiviral activity was identified as Streptomyces parvus by culture characteristics , physiological and biochemical characteristics , and 16 S rDNA sequence analysis . Also ,the influence of different fermentation conditions on antiviral activity of the fermentation broth was studied .The experiment results indicated that the antiviral activities of the fermentation broth was optimal when the strain age was 24 h,inoculum was 8%,temperature was 28℃,initial pH was 7.0,rotary shaker was at 200 r/min.And the vi-rus inhibition rate was above 86%by tobacco mosaic virus ( TMV) necrosis test .