CAJ | 학술논문

通过初筛从健康青年鸡肠道内容物中分离到67株产蛋白酶菌株，采用酪蛋白平板法进行复筛，并对P-98菌株进行形态特征观察、生理生化试验和16S rDNA基因的序列分析。将该菌株的形态特征和生理生化特性鉴定结果与相应种、属模式菌的性状比对，菌株P-98与芽孢杆菌的相应性状相近。根据16 S rDNA基因序列相似性分析，此菌株与Bacillus methylotrophicus标准菌株FZ42相似度达99．78％，因此鉴定该菌株P-98为Bacillus methylotrophicus。
통과초사종건강청년계장도내용물중분리도67주산단백매균주，채용락단백평판법진행복사，병대P-98균주진행형태특정관찰、생리생화시험화16S rDNA기인적서렬분석。장해균주적형태특정화생리생화특성감정결과여상응충、속모식균적성상비대，균주P-98여아포간균적상응성상상근。근거16 S rDNA기인서렬상사성분석，차균주여Bacillus methylotrophicus표준균주FZ42상사도체99．78％，인차감정해균주P-98위Bacillus methylotrophicus。
This experiment was conducted to get a strong vitality of the bacterium strain producing protease . Through screening from healthy young chicken intestinal contents were isolated from 67 strains of protease producing strain,and complex sieve used casein plate method .Then the strain P-98 was characterized by morphological and culture features observation ,physiological and biochemical experiments ,and 16S rDNA gene sequence analysis .As the morphology characteristics and physiological and biochemical of the stain were similar to Bacillus and the simi-larity of the 16S rDNA gene sequences between strain P-98 and the type strain was up to 99.78%,the strain P-98 was initially identified as Bacillus methylotrophicus.