CAJ | 학술논문

人工低温条件下比较了EC1、北京截头和SWCC123种黄瓜苗期在形态、生理及PSⅡ对低温响应的差异。结果表明：低温胁迫均使3种黄瓜产生冷害， EC1植株脱水最轻， SWCC12冷害最严重；3种黄瓜的电渗率和丙二醛（ MDA）因低温处理而增加，变化最小的是EC1，最大的是SWCC12；低温处理提高了3种黄瓜中的超氧化物歧化酶（SOD）活性，尤其是EC1和北京截头的SOD活性反应更为高效和快速，EC1中过氧化物酶（POD）和抗坏血酸过氧化物酶（ APX）呈先增加后降低趋势，但始终高于处理前的，而北京截头中分别先降低后升高及变化不明显，SWCC12中主要变化趋势为增加，EC1体内的过氧化氢酶（ CAT）在低温处理2 d时活性最高，之后下降至原来水平，北京截头和SWCC12中变化不大；3种黄瓜体内可溶性蛋白在胁迫处理时都呈先升高后降低趋势；EC1和北京截头中的可溶性糖也表现出先升高后降低现象，但都显著高于处理前，而SWCC12中可溶性糖呈显著下降趋势；3个基因型黄瓜内叶绿素含量和类胡萝卜素含量随胁迫时间先增加后降低，EC1中维持高含量时间长，下降幅度最小，SWCC12下降幅度最大；低温均影响了3份黄瓜的PSⅡ、Fv/Fm、Yield和ETR，下降幅度最大的是SWCC12、最小的是北京截头。
인공저온조건하비교료EC1、북경절두화SWCC123충황과묘기재형태、생리급PSⅡ대저온향응적차이。결과표명：저온협박균사3충황과산생냉해， EC1식주탈수최경， SWCC12냉해최엄중；3충황과적전삼솔화병이철（ MDA）인저온처리이증가，변화최소적시EC1，최대적시SWCC12；저온처리제고료3충황과중적초양화물기화매（SOD）활성，우기시EC1화북경절두적SOD활성반응경위고효화쾌속，EC1중과양화물매（POD）화항배혈산과양화물매（ APX）정선증가후강저추세，단시종고우처리전적，이북경절두중분별선강저후승고급변화불명현，SWCC12중주요변화추세위증가，EC1체내적과양화경매（ CAT）재저온처리2 d시활성최고，지후하강지원래수평，북경절두화SWCC12중변화불대；3충황과체내가용성단백재협박처리시도정선승고후강저추세；EC1화북경절두중적가용성당야표현출선승고후강저현상，단도현저고우처리전，이SWCC12중가용성당정현저하강추세；3개기인형황과내협록소함량화류호라복소함량수협박시간선증가후강저，EC1중유지고함량시간장，하강폭도최소，SWCC12하강폭도최대；저온균영향료3빈황과적PSⅡ、Fv/Fm、Yield화ETR，하강폭도최대적시SWCC12、최소적시북경절두。
The responses of seedling growth ,physiological properties and PSⅡto low temperature among three genotype cucumbers were comparatively studied .The results showed that:Chilling injury of three genotypes pro-duced under low temperature stress , the dehydrate and chilling injury of EC 1 was the most light , and SWCC12 showed the serious .The contents of Malondialdehyde ( MDA) and electrolyte leakage in three cucumbers increased under cold treatment,the increase extent of two indices in EC1 was minimum,and in SWCC12 was maximal.Super-oxide dismutase ( SOD) in three cucumbers increased during treatment ,especially in EC1 and Beijingjietou SOD were induced rapidly and efficiently .The change of peroxidase ( POD) and ascorbate peroxidase ( APX) activity in EC1 rose first and then fell ,but were higher than pretreatment ,and the contents of two enzymes in Beijingjietou first decreased the increased and had no significant change .While the activity of POD and APX in SWCC 12 increased under low temperature stress.Catalase(CAT) activity in EC1 could be activated by low temperature,reaching the peak in 2 d and then dropped to the former level , that in Beijingjietou and SWCC 12 had not changed much .The content of soluble protein increased before decreasing in three genotypes .And the change of soluble sugar in EC 1 and Beijingjietou as the same as that protein ,and were higher than pretreatment ,but in SWCC12 declined during stress.The contents of chlorophyll and carotenoid in three genotypes first increased and then decreased under low temperature.The high contents of two pigments in EC1 maintained longer time, declined slowly, while that in SWCC12 was contrary.Low temperature hurt the PSⅡ of cucumbers,the Fv/Fm,Yield and ETR in SWCC12 di-clined most seriously ,while in Beijngjietou most slightly .