CAJ | 학술논문

以穿刺短体线虫、咖啡短体线虫、伤残短体线虫、斯克里布纳短体线虫和落选短体线虫5种短体线虫的18个种群为试验材料，将其测序获得的ITS序列与GenBank中已提交的短体线虫序列进行了Blast比对和系统发育分析，以验证核糖体ITS序列作为DNA条形码的可行性。结果显示，核糖体ITS序列作为DNA条形码可以很好的用于鉴别以上5种短体线虫。
이천자단체선충、가배단체선충、상잔단체선충、사극리포납단체선충화락선단체선충5충단체선충적18개충군위시험재료，장기측서획득적ITS서렬여GenBank중이제교적단체선충서렬진행료Blast비대화계통발육분석，이험증핵당체ITS서렬작위DNA조형마적가행성。결과현시，핵당체ITS서렬작위DNA조형마가이흔호적용우감별이상5충단체선충。
The root lesion nematodes,Pratylenchus Flipjev,1936,are among the widespread and seriously de-structive plant endoparasites around the world .Due to the instability of morphological characteristics ,it was very dif-ficult to identify this kind of nematodes just by their morphology .In this study ,18 populations of the following 5 Pra-tylenchus species,i.e.Pratylenchus penetrans,P.coffeae,P.vulnus,P.scribneri and P.neglectus, were used as ex-periment materials ,and their ribosomal ITS sequences were obtained to conduct blast alignment and phylogenetic a -nalysis with other ribosomal ITS sequences of Pratylenchus species submitted in GenBank in order to verify the fea-sibility of ITS regions as DNA barcode .The results showed that DNA barcoding approach using ribosomal ITS re-gions as target fragment could be readily used for identification of the above-mentioned 5 Pratylenchus species .