华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2013年
6期
111-114
,共4页
杨敏%史兆国%韩吉龙%岳耀敬%郭婷婷%郭健%刘建斌%孙晓萍%王朝风%杨博辉
楊敏%史兆國%韓吉龍%嶽耀敬%郭婷婷%郭健%劉建斌%孫曉萍%王朝風%楊博輝
양민%사조국%한길룡%악요경%곽정정%곽건%류건빈%손효평%왕조풍%양박휘
藏羊%低氧诱导因子-1α(HIF-1α)基因%高海拔低氧适应性%单核苷酸多态性
藏羊%低氧誘導因子-1α(HIF-1α)基因%高海拔低氧適應性%單覈苷痠多態性
장양%저양유도인자-1α(HIF-1α)기인%고해발저양괄응성%단핵감산다태성
Tibetan sheep%HIF-1αgene%Adaptation to high-altitude hypoxia%Single nucleotide polymorphism
为了研究低氧诱导因子-1α（ HIF-1α）基因与高海拔低氧适应的相关性，使用高分辨率熔解曲线分析（ HRM）小片段法分型技术对112头藏羊（海拔高于3000 m）和117头低海拔地区羊（海拔低于500 m）HIF-1α基因的G901A位点进行基因分型和分析。结果发现：HIF-1α基因的G901 A位点在2个群体中均处Hardy-Weinberg平衡，基因型频率在高海拔和低海拔群体间均具有统计学差异（P＜0．01）。其中在该位点G/G、G/A、A/A这3种基因型的构成在藏羊上分别为41．07％，46．43％，12．5％，而低海拔群体为66．67％，27．35％，5．98％，藏羊等位基因G、A的分布频率为64．29％和35．71％，而低海拔群体为81．34％，19．66％，HIF-1α基因在G901A位点的基因型和等位基因频率在2组之间比较差异有统计学意义（P＜0．01）。研究结果显示，HIF-1α基因的G901A位点多态性与高海拔低氧适应相关， AA基因型可能更有利于低氧环境。
為瞭研究低氧誘導因子-1α（ HIF-1α）基因與高海拔低氧適應的相關性，使用高分辨率鎔解麯線分析（ HRM）小片段法分型技術對112頭藏羊（海拔高于3000 m）和117頭低海拔地區羊（海拔低于500 m）HIF-1α基因的G901A位點進行基因分型和分析。結果髮現：HIF-1α基因的G901 A位點在2箇群體中均處Hardy-Weinberg平衡，基因型頻率在高海拔和低海拔群體間均具有統計學差異（P＜0．01）。其中在該位點G/G、G/A、A/A這3種基因型的構成在藏羊上分彆為41．07％，46．43％，12．5％，而低海拔群體為66．67％，27．35％，5．98％，藏羊等位基因G、A的分佈頻率為64．29％和35．71％，而低海拔群體為81．34％，19．66％，HIF-1α基因在G901A位點的基因型和等位基因頻率在2組之間比較差異有統計學意義（P＜0．01）。研究結果顯示，HIF-1α基因的G901A位點多態性與高海拔低氧適應相關， AA基因型可能更有利于低氧環境。
위료연구저양유도인자-1α（ HIF-1α）기인여고해발저양괄응적상관성，사용고분변솔용해곡선분석（ HRM）소편단법분형기술대112두장양（해발고우3000 m）화117두저해발지구양（해발저우500 m）HIF-1α기인적G901A위점진행기인분형화분석。결과발현：HIF-1α기인적G901 A위점재2개군체중균처Hardy-Weinberg평형，기인형빈솔재고해발화저해발군체간균구유통계학차이（P＜0．01）。기중재해위점G/G、G/A、A/A저3충기인형적구성재장양상분별위41．07％，46．43％，12．5％，이저해발군체위66．67％，27．35％，5．98％，장양등위기인G、A적분포빈솔위64．29％화35．71％，이저해발군체위81．34％，19．66％，HIF-1α기인재G901A위점적기인형화등위기인빈솔재2조지간비교차이유통계학의의（P＜0．01）。연구결과현시，HIF-1α기인적G901A위점다태성여고해발저양괄응상관， AA기인형가능경유리우저양배경。
To investigate the association between the G 901 A polymorphism of HIF-1αgene and adaptation to high-altitude hypoxia ,the G901 A of HIF-1αgene were genotyped using high resolution melting analysis in 112 un-related healthy Tibetan sheep ( Higher than 3 000 m altitude ) and 117 low altitude sheep ( Lower than 500 m alti-tude).Our results showed all the genotypes of the loci were significantly different between the two groups ( P <0.01).The frequencies of genotypes G/G,G/A and A/A at HIF-1α901 locus in Tibetan sheep were 41.07%, 46.43% and 12.5%,respectively in low altitude sheep ,they were 66.67%,27.35%and 5.98%.Whilst in Tibet-an sheep allele frequencies of G and A were 64.29%and 35.71%,respectively but they were 81.34%and 19.66%respectively in the low altitude sheep (P<0.01).Our results indicate that the polymorphism of genotype in the G901 A of HIF-1αgene were associated with adaptation to high-altitude hypoxia .The sheep with AA genotype may be more conducive to hypoxia .
