CAJ | 학술논문

为了观察不同剂量食欲素A在不同时间对绵羊黄体化颗粒细胞OX1R表达的影响，在绵羊黄体化颗粒细胞中添加不同剂量（0．05，0．2，0．5，1μg/mL）食欲素A，分别于刺激后0，2，6，12 h提取细胞总RNA，运用RT-qPCR方法检测了OX1R mRNA相对表达量变化。结果表明，OX1R mRNA分布于绵羊黄体化颗粒细胞中；添加高剂量（1μg/mL）食欲素A在2 h显著性促进OX1R mRNA表达（P＜0．01），随后这种作用会逐渐减弱；添加低剂量（0.05，0．2，0．5μg/mL）食欲素A后12 h时会显著促进OX1R mRNA表达（P＜0．01），且0．2μg/mL剂量组促进作用更为显著。食欲素A诱导并上调OX1R mRNA的表达，高剂量（1μg/mL）食欲素A可以迅速诱导其表达，而低剂量（0．05，0．2，0．5μg/mL）食欲素A诱导作用较为缓慢，且高剂量（1μg/mL）组食欲素A同低剂量（0．05，0．2，0．5μg/mL）组相比，上调作用更加迅速。
위료관찰불동제량식욕소A재불동시간대면양황체화과립세포OX1R표체적영향，재면양황체화과립세포중첨가불동제량（0．05，0．2，0．5，1μg/mL）식욕소A，분별우자격후0，2，6，12 h제취세포총RNA，운용RT-qPCR방법검측료OX1R mRNA상대표체량변화。결과표명，OX1R mRNA분포우면양황체화과립세포중；첨가고제량（1μg/mL）식욕소A재2 h현저성촉진OX1R mRNA표체（P＜0．01），수후저충작용회축점감약；첨가저제량（0.05，0．2，0．5μg/mL）식욕소A후12 h시회현저촉진OX1R mRNA표체（P＜0．01），차0．2μg/mL제량조촉진작용경위현저。식욕소A유도병상조OX1R mRNA적표체，고제량（1μg/mL）식욕소A가이신속유도기표체，이저제량（0．05，0．2，0．5μg/mL）식욕소A유도작용교위완만，차고제량（1μg/mL）조식욕소A동저제량（0．05，0．2，0．5μg/mL）조상비，상조작용경가신속。
To observe the effect of orexin A on the expression of OX 1 R in ovine luteinized granulosa cells in vitro,the cells were treated with different concentrations (0.05,0.2,0.5,1 μg/mL) of orexins A.Then the total RNA was extracted from the cells after treated in 0,2,6,12 h,and the mRNA expression levels of OX1R were eval-uated using reverse transcription quantitative PCR .In result,orexin A induced release of OX1R in ovine luteinized granulosa cells.In high concentration (1 μg/mL) group,the OX1R mRNA expression level was increased to the highest in 2 h(P<0.01) and gradually weakened since then;in low concentration(0.05,0.2,0.5μg/mL) group, the OX1R mRNA expression level was increased in 12 h and the expression level of 0.2 μg/mL group was in-creased to the highest in 12 h(P<0.01).Orexin A induced expressions of OX1R mRNA,the group of 1 μg/mL was more rapid up regulation compared with the low concentration (0.05,0.2,0.5 μg/mL) group.