CAJ | 학술논문

利用电子克隆技术获得1个辣椒GH3同源基因的cDNA序列，采用生物信息学方法，对该基因所编码的氨基酸从理化性质、分子进化、跨膜结构域、高级结构等多角度进行分析。结果表明，该基因cDNA全长2140 bp，其开放阅读框为1791 bp，编码595个氨基酸。编码蛋白含32个磷酸化位点，无信号肽，无跨膜区域，是定位在叶绿体上的亲水性蛋白。通过氨基酸序列比对发现，辣椒GH3蛋白与拟南芥、大豆、烟草、番茄等植物中的GH3蛋白同源性较高。为该基因的基因功能研究提供了理论依据。
이용전자극륭기술획득1개랄초GH3동원기인적cDNA서렬，채용생물신식학방법，대해기인소편마적안기산종이화성질、분자진화、과막결구역、고급결구등다각도진행분석。결과표명，해기인cDNA전장2140 bp，기개방열독광위1791 bp，편마595개안기산。편마단백함32개린산화위점，무신호태，무과막구역，시정위재협록체상적친수성단백。통과안기산서렬비대발현，랄초GH3단백여의남개、대두、연초、번가등식물중적GH3단백동원성교고。위해기인적기인공능연구제공료이론의거。
A homologous gene of GH3 was obtained in Capsicum annuum by in silicon cloning ,and some char-acters of the gene and its encoded protein were analyzed using the methods of bioinformatics in the following as -pects,including the general physical and chemical properties ,hydrophobicity ,three dimensional structure ,subcellu-lar localization and phylogenetic relationships .The results showed the cDNA length of the gene was 2 140 bp long and contained a complete ORF ( 1 791 bp ) which encoded 595 amino acids .The deduced protein comprised 32 phosphorylation sites ,no signal peptide and transmembrane helix .The GH3 of Capsicum annuum was a hydrophilic and chloroplast protein .The amino acid sequence of the gene showed high similarity with the homologous in other plants,such as Arabidopsis thaliana,Glycine max,Solanum lycopersicum,Nicotiana tabacum.The results laid a solid foundation for gene function analysis of GH3 in Capsicum annuum.