CAJ | 학술논문

目的：探讨反转缝合与丰富环境联合干预对单眼形觉剥夺成年弱视大鼠视皮层可塑性的再激活作用。方法将14日龄大鼠随机分为8组：1~3组为正常对照组,4~8组为单眼剥夺组。剥夺组在生后14天行右侧眼睑缝合制备单眼剥夺模型，放于实验室标准环境下饲养，1~3组、4~6组分别于生后45天、60天、90天处死；7~8组于60日龄时打开剥夺眼，缝合左侧眼睑行遮盖治疗，第7组放于标准环境中饲养30d，即反转缝合组，第8组放于丰富环境中饲养30天，即反转缝合联合丰富环境干预组，此两组至90日龄处死取材；免疫组化染色检测并分析各组大鼠视皮层PSD-95蛋白表达水平的变化。结果同年龄段弱视组视皮层PSD-95呈阳性神经元密度降低(P<0.001)；同年龄段反转缝合干预组视皮层较弱视组PSD-95呈阳性神经元密度增加(P<0.05)，但仍低于正常组(P<0.05)；同年龄段反转缝合、结合丰富环境干预组视皮层与弱视组、反转缝合组相比，PSD-95呈阳性神经元密度增加(P<0.05)，与正常组相比，其差异无统计学意义(P>0.05)。结论单眼剥夺影响了关键期后成年大鼠视皮层PSD-95的表达，提示PSD-95可能参与调节成年视皮层可塑性；反转缝合单纯干预、反转缝合联合丰富环境干预能部分重新激活剥夺性成年弱视大鼠视皮层可塑性，反转缝合与丰富环境联合干预的再激活作用更为明显。
목적：탐토반전봉합여봉부배경연합간예대단안형각박탈성년약시대서시피층가소성적재격활작용。방법장14일령대서수궤분위8조：1~3조위정상대조조,4~8조위단안박탈조。박탈조재생후14천행우측안검봉합제비단안박탈모형，방우실험실표준배경하사양，1~3조、4~6조분별우생후45천、60천、90천처사；7~8조우60일령시타개박탈안，봉합좌측안검행차개치료，제7조방우표준배경중사양30d，즉반전봉합조，제8조방우봉부배경중사양30천，즉반전봉합연합봉부배경간예조，차량조지90일령처사취재；면역조화염색검측병분석각조대서시피층PSD-95단백표체수평적변화。결과동년령단약시조시피층PSD-95정양성신경원밀도강저(P<0.001)；동년령단반전봉합간예조시피층교약시조PSD-95정양성신경원밀도증가(P<0.05)，단잉저우정상조(P<0.05)；동년령단반전봉합、결합봉부배경간예조시피층여약시조、반전봉합조상비，PSD-95정양성신경원밀도증가(P<0.05)，여정상조상비，기차이무통계학의의(P>0.05)。결론단안박탈영향료관건기후성년대서시피층PSD-95적표체，제시PSD-95가능삼여조절성년시피층가소성；반전봉합단순간예、반전봉합연합봉부배경간예능부분중신격활박탈성성년약시대서시피층가소성，반전봉합여봉부배경연합간예적재격활작용경위명현。
Objective To investigate effect of reverse suture combined with enriched environment intervention on the reactivation of ocular dominance plasticity in the visual cortex of monocular form-deprived adult rats. Methods The 14-day-old rats were randomly divided into eight groups: 1~3 groups are normal control group (NC) , 4~8 groups are monocular deprivation group (MD) .The right eyelids of MD groups were sutured at postnatal 14 days to obtain animal model. Rats were housed in the standard environment. All the rats of 1~6 groups were sacrificed at postnatal 45, 60 and 90 days. The right eyelids of the rats for 7~8 groups were opened while left eyelids were sutured at postnatal 45 days.Rats of 7 group ( RS) were housed in the standard environment and rats of 8 group ( RS+EE) were housed in the enriched environment. The last two groups were sacrificed at postnatal 90 days. We measured the expression of PSD-95 protein in the visual cortex of the different groups by using immunohistochemistry staining. Results The density of positive neurons of PSD-95 in the visual cortex of MD group was significantly much less than those in NC groups at the same age ( P< 0.001) .The density of positive neurons of PSD-95 in RS group was higher than those of MD group (P< 0.05),but still lower than those of NC group at the same age (P< 0.05) . The density of positive neurons of PSD-95 in RS+EE group was higher than those of MD group and RS group at the same age ( P <0.05) , however, compared with NC group, the difference was not statistically significant ( P > 0.05) . Conclusions Monocular deprivation might influence the expression of PSD-95 in the visual cortex of monocular deprived form adult rats after the critical period, The results implied that PSD-95 might participated in the ocular dominance plasticity in the adult visual cortex. Effect of re verse suture combined with enriched environment intervention was much stronger than reverse suture alone intervention on the reactivation of ocular dominance plasticity in the visual cortex of monocular form deprived adult rats.