CAJ | 학술논문

目的：探讨回阳生肌膏治疗慢性皮肤溃疡腐肉、肉芽、脓液及组织HE染色形态变化规律。方法收集首都医科大学附属北京中医医院2012年11月~2013年11月40例局部辨证为寒湿凝滞证慢性疮面患者，随机分为两组，治疗组应用回阳生肌膏纱条，对照组按照常规方法进行。观察两组治疗前后疮面形态评分和治疗前后皮下组织HE染色变化情况。结果对照组总的评分治疗前为(29.1±5.7)分，治疗组为(28.4±6.1)分，差异无统计学意义(P>0.05)；对照组治疗后为(25.7±5.4)分，与治疗前比较，差异有统计学意义(P<0.05)，治疗组为(22.9±6.8)分，与治疗前比较，差异有统计学意义(P<0.05)。对照组与治疗组治疗后组间比较，差异有统计学意义(P<0.05)。皮下组织HE染色观察，治疗前炎症细胞较多，内皮细胞、成纤维细胞较少且形态瘦小，分布散乱，胶原纤维淡染，治疗后炎症细胞减少，内皮细胞、成纤维细胞数量多形态饱满，分布规律，胶原纤维深染，治疗组较对照组有更好的愈合趋势。结论回阳生肌膏主要功效“温阳益气”，使慢性皮肤溃疡疮面由“阴”转“阳”，由“静”转“动”，从而加速疮面愈合。
목적：탐토회양생기고치료만성피부궤양부육、육아、농액급조직HE염색형태변화규률。방법수집수도의과대학부속북경중의의원2012년11월~2013년11월40례국부변증위한습응체증만성창면환자，수궤분위량조，치료조응용회양생기고사조，대조조안조상규방법진행。관찰량조치료전후창면형태평분화치료전후피하조직HE염색변화정황。결과대조조총적평분치료전위(29.1±5.7)분，치료조위(28.4±6.1)분，차이무통계학의의(P>0.05)；대조조치료후위(25.7±5.4)분，여치료전비교，차이유통계학의의(P<0.05)，치료조위(22.9±6.8)분，여치료전비교，차이유통계학의의(P<0.05)。대조조여치료조치료후조간비교，차이유통계학의의(P<0.05)。피하조직HE염색관찰，치료전염증세포교다，내피세포、성섬유세포교소차형태수소，분포산란，효원섬유담염，치료후염증세포감소，내피세포、성섬유세포수량다형태포만，분포규률，효원섬유심염，치료조교대조조유경호적유합추세。결론회양생기고주요공효“온양익기”，사만성피부궤양창면유“음”전“양”，유“정”전“동”，종이가속창면유합。
Objective To investigate the treatment of Huiyang Shengji Ointment to chronic skin ulcer carrion, granula-tion, pus and the change regulations of tissue HE staining. Methods 40 patients in Beijing TCM Hospital Affiliated to Capital Medical University from November 2012 to November 2013 whose local syndrome differentiation were cold damp stagnation syndrome of chronic wound, the subjects were randomly divided into two groups. The treatment group was used Huiyang Shengji Ointment gauze, the control group was taken conventional method. The wound surface mor-phology scoring and the two groups' changes of subcutaneous tissue HE staining before and after treatment were ob-served. Results The total score of the control group was (29.1±5.7) scores, and the treatment group was (28.4±6.1) scores before treatment, the difference between the two groups showed no significant difference (P > 0.05); the total score of the control group was (25.7±5.4) scores after the treatment, compared with the control group, the difference was statistically significant (P<0.05). The total score of the treatment group was (22.9±6.8) scores, compared with the treat-ment group, the difference was statistically significant (P < 0.05). The comparison between the control group and the treatment group showed that, the difference was statistically significant (P<0.05). The subcutaneous tissue HE staining showed that there were more inflammatory cells before treatment, endothelial cells and fibroblasts were less and thin, and they sprawled out in some areas, collagen fibers were dyed pale; there were less inflammatory cells after treatment, endothelial cells and fibroblasts were more and well-stacked, they distribute regularly, ollagen fibers were dyed dark. Healing trend was better in the treatment group than in the control group. Conclusion Warming Yang and Invigorating Qi, the main effect of Huiyang Shengji Ointment, can make chronic skin ulcer w ound turn "Yin" into "Yang", and turn "Static" into "Dynamic", so that the speed of wound healing will be accelerated.