CAJ | 학술논문

目的：探讨糖心乐(TXL)对糖尿病心肌病大鼠心肌酶、心肌转化生长因子β1(TGF-β1)表达的影响。方法SD大鼠腹腔注射链脲佐菌素建立糖尿病模型，8周后形成糖尿病心肌病模型，随机分为模型对照组、达美康对照组、TXT大、小剂量组，各组大鼠均为12只，另设正常SD大鼠10只作为正常对照组。达美康对照组、TXL大、小剂量组分别给予3 mg/mL达美康混悬液、2 g/mL TXL、1 g/mL TXL灌胃治疗，模型对照组给予生理盐水灌胃。治疗4周后处死，取血检测血糖、肌酸激酶(CK)、乳酸脱氢酶(LDH)，切取心肌组织，免疫组化法检测TGF-β1表达情况。结果与模型对照组比较，TXL大剂量组血糖明显降低[(10.54±2.06)mmol/L比(23.16±5.74)mmol/L]，CK、LDH明显升高[(120.57±18.83)U/mg比(78.26±12.01)U/mg，(5052.06±418.64)U/mg比(3784.15±349.62)U/mg]；TGF-β1阳性染色颗粒的平均光密度(MOD)降低[(0.148±0.006)比(0.262±0.008)]；差异均有高度统计学意义(均P约0.01)。结论 TXL对糖尿病大鼠有一定的降血糖作用，可减少心肌酶漏出，改善心肌能量代谢，同时降低心肌中TGF-β1的表达，减慢心肌组织纤维化进程，延缓和改善糖尿病心肌病的心肌损伤。
목적：탐토당심악(TXL)대당뇨병심기병대서심기매、심기전화생장인자β1(TGF-β1)표체적영향。방법SD대서복강주사련뇨좌균소건립당뇨병모형，8주후형성당뇨병심기병모형，수궤분위모형대조조、체미강대조조、TXT대、소제량조，각조대서균위12지，령설정상SD대서10지작위정상대조조。체미강대조조、TXL대、소제량조분별급여3 mg/mL체미강혼현액、2 g/mL TXL、1 g/mL TXL관위치료，모형대조조급여생리염수관위。치료4주후처사，취혈검측혈당、기산격매(CK)、유산탈경매(LDH)，절취심기조직，면역조화법검측TGF-β1표체정황。결과여모형대조조비교，TXL대제량조혈당명현강저[(10.54±2.06)mmol/L비(23.16±5.74)mmol/L]，CK、LDH명현승고[(120.57±18.83)U/mg비(78.26±12.01)U/mg，(5052.06±418.64)U/mg비(3784.15±349.62)U/mg]；TGF-β1양성염색과립적평균광밀도(MOD)강저[(0.148±0.006)비(0.262±0.008)]；차이균유고도통계학의의(균P약0.01)。결론 TXL대당뇨병대서유일정적강혈당작용，가감소심기매루출，개선심기능량대사，동시강저심기중TGF-β1적표체，감만심기조직섬유화진정，연완화개선당뇨병심기병적심기손상。
Objective To explore the effects of Tangxinle (TXL) on myocardial enzyme and transforming growth factor-β1 (TGF-β1) in experimental rats with diabetic cardiomyopathy. Methods Diabetes model was established with streptozotocin by intraperitoneal injection in Sprague-Dawley rats, after 8 weeks diabetes cardiomyopathy model was formed, then diabetic cardiomyopathy rats were randomly divided into four groups: model control group, the Diamicron control group, TXL high-dose group, TXL small-dose group, 12 rats in each group, 10 normal rats were as the normal control group. Diamicron control group, TXL high-dose group, TXL small-dose group were given 3 mg/mL of Diamicron suspension, 2 g/mL TXL, 1 g/mL TXL lavage treatment respectively, the model control group was given normal saline lavage. Rats of five groups were killed after 4 weeks treatment. Blood glucose, creatine kinase (CK) and lactic dehydrogenase (LDH) were detected, and the expression of TGF-β1 in five groups were detected by immunohistochemistry method. Results Compared with model control group, TXL high-dose group fasting blood glucose decreased obviously [(10.54±2.06) mmol/L vs (23.16±5.74) mmol/L];CK and LDH increased remarkably [(120.57±18.83) U/mg vs (78.26±12.01) U/mg;(5052.06±418.64) U/mg vs (3784.15±349.62) U/mg];the average absorbance value of staining positive particles TGF-β1 in myocardial cell reduced [(0.148±0.006) vs (0.262±0.008)], the differences were statistically significant (all P<0.01). Conclusion TXL has a certain hypoglycemic effect on diabetic rats, it can reduce the myocardial enzyme leakage to improve the metabolic disorder of energy, meanwhile it can reduce the expression of TGF-β1 to slow down fibrous degeneration in myocardial tissue and delay development of diabetic cardiomyopathy.