CAJ | 학술논문

目的：研究缬沙坦对人冠脉内皮细胞( HCAEC)氧化应激过程中gp91 Phox的影响。方法对照组：体外贴壁培养HCAEC,不进行其他干预；实验组：相同培养条件下加入缬沙坦(10μmol/L)培养24 h。应用Western blot法和细胞免疫荧光法测定两组细胞中gp91 Phox的水平,对两组结果进行统计分析并比较。结果实验组HCAEC中gp91 Phox的水平明显低于对照组,差异有统计学意义( P<0．01)。结论缬沙坦可以显著减少HCAEC中尼克酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶亚基gp91Phox的表达,从而降低氧化应激水平。
목적：연구힐사탄대인관맥내피세포( HCAEC)양화응격과정중gp91 Phox적영향。방법대조조：체외첩벽배양HCAEC,불진행기타간예；실험조：상동배양조건하가입힐사탄(10μmol/L)배양24 h。응용Western blot법화세포면역형광법측정량조세포중gp91 Phox적수평,대량조결과진행통계분석병비교。결과실험조HCAEC중gp91 Phox적수평명현저우대조조,차이유통계학의의( P<0．01)。결론힐사탄가이현저감소HCAEC중니극선알선표령이핵감산린산(NADPH)양화매아기gp91Phox적표체,종이강저양화응격수평。
Objective To study the effect on human coronary artery endothelial cells ( HCAEC ) gp91 Phox in the process of oxidative stress by valsartan. Methods The control group: adherent culture HCAEC in vitro, no other intervention;the experimental group:under the same conditions with valsartan (10μmol/L) train for 24 hours. U-sing Western blot and cell immunofluorescence measure gp91 Phox levels in two groups of cells. The results were sta-tistically analyzed and compared in both groups. Results The experimental group gp91 Phox level significantly lower than the control group in HCAEC( P<0. 01 ) , the difference was statistically significant. Conclusion Valsartan can significantly decrease the nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase subunit gp91 Phox ex-pression in HCAEC, thus reduce oxidative stress level.