CAJ | 학술논문

分别以硝酸盐、亚硝酸盐、氧气为电子受体,采用3组SBR反应器培养除磷污泥,连续126d的稳定运行表明：以硝酸盐、亚硝酸盐、氧气为电子受体除磷污泥对TP平均去除率分别为84.8%,78.7%,87.4%,出水TP平均浓度分别为0.758,0.931,0.632mg/L.采用高通量测序技术对不同电子受体除磷污泥的相似性与菌群结构进行了研究,结果表明,以硝酸盐,亚硝酸盐为电子受体的反硝化除磷污泥具有近似的菌群结构,与好氧除磷污泥菌群结构差异较大.基于各样品主导OTUs序列的系统发育关系及其比例的分布,主导微生物主要可以分为5个簇.通过序列比对,在97%的序列相似度条件下,种泥中聚磷菌与聚糖菌序列比例为0.716%与0.368%,以硝酸盐、亚硝酸盐、氧气为电子受体除磷污泥中聚磷菌与聚糖菌序列比例分别为1.78%,2.53%,4.80%与1.44%,1.32%,30.9%,厌氧-缺氧条件有利于抑制聚糖菌.亚硝酸盐为反硝化除磷污泥电子受体时潜在公共卫生安全隐患.
분별이초산염、아초산염、양기위전자수체,채용3조SBR반응기배양제린오니,련속126d적은정운행표명：이초산염、아초산염、양기위전자수체제린오니대TP평균거제솔분별위84.8%,78.7%,87.4%,출수TP평균농도분별위0.758,0.931,0.632mg/L.채용고통량측서기술대불동전자수체제린오니적상사성여균군결구진행료연구,결과표명,이초산염,아초산염위전자수체적반초화제린오니구유근사적균군결구,여호양제린오니균군결구차이교대.기우각양품주도OTUs서렬적계통발육관계급기비례적분포,주도미생물주요가이분위5개족.통과서렬비대,재97%적서렬상사도조건하,충니중취린균여취당균서렬비례위0.716%여0.368%,이초산염、아초산염、양기위전자수체제린오니중취린균여취당균서렬비례분별위1.78%,2.53%,4.80%여1.44%,1.32%,30.9%,염양-결양조건유리우억제취당균.아초산염위반초화제린오니전자수체시잠재공공위생안전은환.
Phosphorus removal sludge with nitrate, nitrite and oxygen as the sole electron acceptor were acclimatized in three parallel sequencing batch reactors, namely RN03, RN02 and R02 respectively. Operation results showed that phosphorus removal rates were 84.8%, 87.4% and 78.7% on average for RN03, RN02 and R02, with effluent TP concentration of 0.758mg/L, 0.931mg/L and 0.632mg/L. High-throughput technology was employed to evaluate the microbial similarity and community structure of phosphorus removal sludge with different electron acceptors. Results indicated that denitrifying phosphorus removal sludge with nitrate and nitrite as electron acceptors exhibited similar bacterial community structure and was distinct from that of aerobic phosphorus removal sludge. The dominant microbial organisms were clustered into five clades based on the phylogenetic relationship of the significant OTUs sequences. Through sequence BLAST, 0.716%, 1.78%, 2.53% and 4.80% of sequences from Seed, RN03, RN02 and R02 could be assigned toAccumulibacter-like sequences at similarity level of 97%; 0.368%, 1.44%, 1.32% and 30.9% of sequences could be assigned to GAO-like sequences, indicating that alternating anaerobic-anoxic condition was favorable toward elimination of GAO. Moreover, potential public health hazard was revealed for nitrite based denitrifying phosphorus removal sludge.