CAJ | 학술논문

目的：研究β-榄香烯对小鼠肝癌细胞增殖及凋亡的影响。方法：用CCK-8法检测β-榄香烯对肝癌BNL 1ME A.7R.1（BNL）细胞增殖的影响；用Annexin V FITC/PI双染分别进行荧光显微镜观察和流式细胞检测β-榄香烯处理后肝癌BNL细胞凋亡情况。结果：β-榄香烯对肝癌BNL细胞生长具有显著抑制作用，呈浓度和时间依赖性，药物作用早期可诱导细胞凋亡，凋亡率随药物浓度而递增，随着作用时间延长细胞坏死明显增多。结论：β-榄香烯可有效抑制肝癌细胞增殖；其抑制作用与促进细胞凋亡有关。
목적：연구β-람향희대소서간암세포증식급조망적영향。방법：용CCK-8법검측β-람향희대간암BNL 1ME A.7R.1（BNL）세포증식적영향；용Annexin V FITC/PI쌍염분별진행형광현미경관찰화류식세포검측β-람향희처리후간암BNL세포조망정황。결과：β-람향희대간암BNL세포생장구유현저억제작용，정농도화시간의뢰성，약물작용조기가유도세포조망，조망솔수약물농도이체증，수착작용시간연장세포배사명현증다。결론：β-람향희가유효억제간암세포증식；기억제작용여촉진세포조망유관。
This study was aimed to investigate the effect of β-elemene on the proliferation and apoptosis in mouse hepatoma cells (BNL). Cell Counting Kit-8 (CCK-8) was employed to detect the proliferation in BNL 1ME A.7R.1 cell line induced by β-elemene. Flow cytometry and immunofluorescence were introduced to test the apoptosis of β-elemene in BNL. The results showed that the proliferation of BNL was obviously inhibited by β-elemene, which showed concentration and time dependence; while apoptosis of BNL was detected in the prior β-elemene function time, also with a remarkable concentration and time dependence. It was concluded that β-elemene can effectively inhibit BNL proliferation, which might be well related with the apoptosis function of β-elemene.