CAJ | 학술논문

目的：测定柿树寄主的桑寄生科7种不同属种的桑寄生药用植物中槲皮苷含量。方法：采用HPLC法对柿树寄主的桑寄生科7种不同属种桑寄生药用植物槲皮苷含量进行测定，样品采用甲醇超声提取方法制备，用Inertsil ODS-SP 色谱柱（150 mm×4.6 mm，5μm），甲醇-0.1%磷酸溶液（52：48）为流动相，流速1.0 mL·min-1，检测波长254 nm。结果：槲皮苷的线性范围0.21~350.00 mg·L-1（ r =0.9999），平均回收率为98.86%，RSD=2.31%。桑寄生科7种不同属种药用植物茎枝中槲皮苷含量为0.3589~3.6770 mg·g-1，叶中槲皮苷含量为5.0091~76.3813 mg·g-1。结论：桑寄生科不同属种桑寄生药用植物槲皮苷的含量各不相同，主要存在于叶中，而茎枝中的含量较低。
목적：측정시수기주적상기생과7충불동속충적상기생약용식물중곡피감함량。방법：채용HPLC법대시수기주적상기생과7충불동속충상기생약용식물곡피감함량진행측정，양품채용갑순초성제취방법제비，용Inertsil ODS-SP 색보주（150 mm×4.6 mm，5μm），갑순-0.1%린산용액（52：48）위류동상，류속1.0 mL·min-1，검측파장254 nm。결과：곡피감적선성범위0.21~350.00 mg·L-1（ r =0.9999），평균회수솔위98.86%，RSD=2.31%。상기생과7충불동속충약용식물경지중곡피감함량위0.3589~3.6770 mg·g-1，협중곡피감함량위5.0091~76.3813 mg·g-1。결론：상기생과불동속충상기생약용식물곡피감적함량각불상동，주요존재우협중，이경지중적함량교저。
This study was aimed to determine contents of quercitrin in 7 Taxilli Herba parasitized in Diospyros kaki Thunb . The content of quercitrin in Taxilli Herba parasitized in D. kaki Thunb. was determined by HPLC and quercitrin was refluxing extracted using methanol. The analytical column was Inertsil ODS-SP (150 mm í 4.6 mm, 5 μm). The mobile phase was methanol-0.1% phosphate (52:48). The flow rate was 1.0 mL·min-1. The detection wavelength was 254 nm. The results showed that the linear range of quercetin was within the range of 0.21~350.00 mg·L-1 (r = 0.999 9). The average recovery rate was 98.86%, RSD = 2.31%. Contents of quercitrin from 7 kinds of Taxilli Herba parasitized were 0.358 9~3.677 0 mg·g-1 in branches and 5.009 1~76.381 3 mg·g-1 in leaves. It was concluded that contents of quercitrin in different kinds of Taxilli Herba parasitized in D. kaki Thunb. are different, which are mainly found in the leaves. The content in branches is relatively low.