CAJ | 학술논문

目的：探讨高脂血症( HL)影响小鼠睾丸睾酮合成机制及辛伐他汀( SIMV)的干预作用。方法18只C57BL/6J雄性小鼠随机均分为3组：HL 组、SIMV 组和正常对照组( CTRL组)；4个月后测血脂、体重；3β-HSD免疫组化法检测睾丸间质细胞； RT-PCR法和Western blot法检测类固醇激素合成急性调节蛋白( StAR)与细胞色素P450胆固醇侧链裂解酶(P450scc)的表达。结果① HL组和SIMV组体重、总胆固醇( TC )和低密度脂蛋白胆固醇( LDL-C )明显高于CTRL组( P<0．05)；SIMV组体重和TC比HL组显著下降(P<0．05),LDL-C无明显变化。② HL组StAR和P450scc的mRNA与蛋白表达较 CTRL 组显著下调(P <0．05,P <0．01)；SIMV组较HL组则两者表达均升高(P<0．01)。③3β-HSD免疫组化染色显示各组小鼠睾丸间质细胞数差异无统计学意义。结论 SIMV可以改善HL引起的睾丸间质细胞合成睾酮能力下调。
목적：탐토고지혈증( HL)영향소서고환고동합성궤제급신벌타정( SIMV)적간예작용。방법18지C57BL/6J웅성소서수궤균분위3조：HL 조、SIMV 조화정상대조조( CTRL조)；4개월후측혈지、체중；3β-HSD면역조화법검측고환간질세포； RT-PCR법화Western blot법검측류고순격소합성급성조절단백( StAR)여세포색소P450담고순측련렬해매(P450scc)적표체。결과① HL조화SIMV조체중、총담고순( TC )화저밀도지단백담고순( LDL-C )명현고우CTRL조( P<0．05)；SIMV조체중화TC비HL조현저하강(P<0．05),LDL-C무명현변화。② HL조StAR화P450scc적mRNA여단백표체교 CTRL 조현저하조(P <0．05,P <0．01)；SIMV조교HL조칙량자표체균승고(P<0．01)。③3β-HSD면역조화염색현시각조소서고환간질세포수차이무통계학의의。결론 SIMV가이개선HL인기적고환간질세포합성고동능력하조。
Objective To investigate the effect of hyperlipidemia( HL) on the testosterone synthesis of Leydig cells and the protection conferred by simvastatin(SIMV) treatment. Methods Eighteen C57BL/6J male mice were ran-domly divided into hyperlipidemia group ( HL group ) , simvastatin treatment group ( SIMV group ) and control group ( CTRL group) . After 4 months,the body weight and serum lipid profiles were detected. Leydig cells were evaluated by 3β-HSD immunohistochemical staining. Expressions of steroidogenic acute regulatory protein( StAR) and cyto-chrome P450 Cholestero side chain lyase(P450scc)were determined by RT-PCR and Western blot. Results The high-fat diet led to a significant increase in body weight,total cholesterol( TC) and low density lipoprotein cholesterol ( LDL-C ) in HL group and SIMV group as compared to CTRL group ( P <0. 05 ) . A significant decrease in body weight and serum TC was noticed in SIMV group in comparison with HL group ( P<0. 05 ) . The decreased mRNA and protein expressions of testicular StAR and P450 scc were observed in hyperlipidemic mice as compared to CTRL group(P <0. 05, P <0. 01). The StAR and P450scc expressions were significantly improved in SIMV group in comparison with the HL group ( P <0. 01 ) . There were no significant differences of Leydig cells number among these experimental groups. Conclusion SIMV may have a role in protecting Leydig cell function against metabolic damage caused by HL.