海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
4期
55-57
,共3页
黄桂坤%邱莉%刘向红%黄丽凤%李福森%罗艳%谢集照%张若蘅
黃桂坤%邱莉%劉嚮紅%黃麗鳳%李福森%囉豔%謝集照%張若蘅
황계곤%구리%류향홍%황려봉%리복삼%라염%사집조%장약형
番石榴叶%总三萜%紫外分光光度法
番石榴葉%總三萜%紫外分光光度法
번석류협%총삼첩%자외분광광도법
Psidium guajava L.%Total triterpenes%UV Spectrophotometry
目的：建立一种番石榴叶药材中总三萜含量测定的方法，并考察10批番石榴叶药材中总三萜含量。方法以熊果酸为对照品，香草醛-冰醋酸-高氯酸为显色体系，采用紫外分光光度法在最大吸收波长为548nm处测定番石榴叶药材总三萜含量。结果熊果酸在8.13～28.44μg · mL -1范围内线性关系良好，总三萜平均回收率为99.43％，RSD为2.30％。结论该方法操作简便、快速、准确、稳定可靠，可作为番石榴叶药材总三萜含量测定的方法。
目的：建立一種番石榴葉藥材中總三萜含量測定的方法，併攷察10批番石榴葉藥材中總三萜含量。方法以熊果痠為對照品，香草醛-冰醋痠-高氯痠為顯色體繫，採用紫外分光光度法在最大吸收波長為548nm處測定番石榴葉藥材總三萜含量。結果熊果痠在8.13～28.44μg · mL -1範圍內線性關繫良好，總三萜平均迴收率為99.43％，RSD為2.30％。結論該方法操作簡便、快速、準確、穩定可靠，可作為番石榴葉藥材總三萜含量測定的方法。
목적：건립일충번석류협약재중총삼첩함량측정적방법，병고찰10비번석류협약재중총삼첩함량。방법이웅과산위대조품，향초철-빙작산-고록산위현색체계，채용자외분광광도법재최대흡수파장위548nm처측정번석류협약재총삼첩함량。결과웅과산재8.13～28.44μg · mL -1범위내선성관계량호，총삼첩평균회수솔위99.43％，RSD위2.30％。결론해방법조작간편、쾌속、준학、은정가고，가작위번석류협약재총삼첩함량측정적방법。
OBJECTIVE To establish a method for the quality control of Psidium guajava L.and investigate the quality of 10 batches of Psidium guajava L.collected from different places.METHODS Ursolic acid and vanillin-acetic acid-perchloric acid system were employed as reference substance and chromogenic reagent ,respectively,the total triterpenes of Psidium guajava L.was measured by UV spectrophotometry at the 548 nm.RESULTS The linear relationship of ursolic acid was in the range of 8.13~28.44μg· mL-1.The average recovery was 99.43%( RSD=2.30%).CONCLUSION This method is simple ,quick,accurated,stable and reliable,and it could be used to de-termine the total triterpenes of Psidium guajava L.
