济宁医学院学报
濟寧醫學院學報
제저의학원학보
JOURNAL OF JINING MEDICAL COLLEGE
2014年
1期
30-32,36
,共4页
胃癌%Wnt10b%实时荧光定量PCR%Western-blot
胃癌%Wnt10b%實時熒光定量PCR%Western-blot
위암%Wnt10b%실시형광정량PCR%Western-blot
Gastric cancer (GC)%Wnt10b%Real time fluorescent quantitative PCR%Western-blot
目的:检测胃癌及癌旁组织中 Wnt10b 的表达,探讨其与临床病理参数的关系。方法收集25例胃癌患者手术切除的癌组织及对应的癌旁组织,用实时荧光定量 PCR检测 Wnt10b mRNA的表达,Western-blot技术检测 Wnt10b蛋白的表达,分析 Wnt10b mRNA在胃癌组织中的表达与临床病理参数的关系。结果胃癌组织中 Wnt10b mRNA和蛋白表达水平明显高于癌旁组织(P<0.001),胃癌组织中 Wnt10b mRNA 表达阳性率与肿瘤远处转移相关(P<0.05),但与年龄、性别、肿瘤大小、TNM分期、分化程度和浸润深度无关。结论 Wnt10b在胃癌组织中呈活化状态,可能参与胃癌的发生发展。
目的:檢測胃癌及癌徬組織中 Wnt10b 的錶達,探討其與臨床病理參數的關繫。方法收集25例胃癌患者手術切除的癌組織及對應的癌徬組織,用實時熒光定量 PCR檢測 Wnt10b mRNA的錶達,Western-blot技術檢測 Wnt10b蛋白的錶達,分析 Wnt10b mRNA在胃癌組織中的錶達與臨床病理參數的關繫。結果胃癌組織中 Wnt10b mRNA和蛋白錶達水平明顯高于癌徬組織(P<0.001),胃癌組織中 Wnt10b mRNA 錶達暘性率與腫瘤遠處轉移相關(P<0.05),但與年齡、性彆、腫瘤大小、TNM分期、分化程度和浸潤深度無關。結論 Wnt10b在胃癌組織中呈活化狀態,可能參與胃癌的髮生髮展。
목적:검측위암급암방조직중 Wnt10b 적표체,탐토기여림상병리삼수적관계。방법수집25례위암환자수술절제적암조직급대응적암방조직,용실시형광정량 PCR검측 Wnt10b mRNA적표체,Western-blot기술검측 Wnt10b단백적표체,분석 Wnt10b mRNA재위암조직중적표체여림상병리삼수적관계。결과위암조직중 Wnt10b mRNA화단백표체수평명현고우암방조직(P<0.001),위암조직중 Wnt10b mRNA 표체양성솔여종류원처전이상관(P<0.05),단여년령、성별、종류대소、TNM분기、분화정도화침윤심도무관。결론 Wnt10b재위암조직중정활화상태,가능삼여위암적발생발전。
Objective This study aimed to detect the expression of Wnt10b in paired tumor and non-cancerous tissues of gastric cancer (GC)patients and explore correlation of Wnt10b mRNA expression with the clinicopatho-logical parameters of GC.Methods The surgically resected paired cancerous and adjacent non-cancerous tissues of 25 GC patients were collected.The expression of Wnt10b mRNA was examined by Quantitative real-time PCR (qRT-PCR).Western-blot was employed to detect Wnt10b protein levels.Correlation between Wnt10b mRNA ex-pression and clinicopathological parameters was analyzed.Results The levels of Wnt10b mRNA in cancerous tis-sues were higher compared with matching adjacent non-cancerous tissues (P<0.001 ).The protein levels of Wnt10b were consistent with the expression levels of Wnt10b mRNA.Wnt10b mRNA expression was correlated with the lymph node metastasis of tumor (P<0.05),but no association was found between Wnt10b mRNA expres-sion and other clinicopathological parameters including age,gender,tumor size,TNM stage,differentitation stage and depth of invasion.Conclusion The expression levels of Wnt10b in cancerous tissues were higher than adjacent non-cancerous tissues,and there was correlation between the expression levels of Wnt10b and the lymph node me-tastasis of tumor.