CAJ | 학술논문

[目的]探索一种牛眼球的石蜡切片制作方法。[方法]试验对照组采用常规石蜡切片制作，试验组采用"角膜开窗再固定法"制作石蜡切片。[结果]试验组标本固定后肉眼可见视网膜与脉络膜紧密相连，仅有小部分视网膜脱离的现象；切片显示小梁网连续性未被破坏、眼球壁全层组织连续性未破坏、各层未出现分离现象；视网膜无脱离、各层组织细胞无收缩现象。说明试验组大大改进了眼球固定、脱水、透明技术。[结论]为眼球研究和相应病理研究提供基础。
[목적]탐색일충우안구적석사절편제작방법。[방법]시험대조조채용상규석사절편제작，시험조채용"각막개창재고정법"제작석사절편。[결과]시험조표본고정후육안가견시망막여맥락막긴밀상련，부유소부분시망막탈리적현상；절편현시소량망련속성미피파배、안구벽전층조직련속성미파배、각층미출현분리현상；시망막무탈리、각층조직세포무수축현상。설명시험조대대개진료안구고정、탈수、투명기술。[결론]위안구연구화상응병리연구제공기출。
[Objective] This study aimed to establish a new method for preparing paraffin sections of cattle eyebal s. [Method] The conventional method was used to prepare paraffin sections for cattle eyebal s in the control and a new method termed"opening a window on cornea and refixation" was used to prepare paraffin sections for cattle eyebal s in the treatment group. [Result] After the prepared specimens in the treatment group were fixed, it could be macroscopical y observed that retina and choroid were closely connected, with detachment occurring at a smal portion be-tween the two. According to the paraffin sections, it was microscopical y observed that the continuity of trabecular meshwork was intact, as wel as the continuity be-tween different layers of eyebal wal , without detachment between them, no retinal detachment, no shrinkage of each layer of tissue cells. [Conclusion] This study pro-vides a foundation for the basic research and pathological study of eyebal s.