CAJ | 학술논문

[目的]从分子水平上了解贵州省甘蓝型油菜主推品种的遗传多样性。[方法]利用国家油菜区域试验 DNA指纹鉴定使用的40对 SSR引物对贵州省9个甘蓝型油菜主推品种进行多态性检测，并进行聚类分析。[结果]试验共扩增出条带191个，其中多态性条带143个，多态性比率为75%。经聚类分析，9个油菜品种被分为 A、B2簇，其中 A簇包含7个品种，B簇为贵州省油料研究所选育的2个品种。 A簇在相似系数0.6434处又分为 A1和 A22个亚簇，A1亚簇为贵州省油菜研究所选育的3个品种，A2亚簇为贵州省油料研究所选育4个品种。[结论]同一单位选育出的品种都有更为接近的遗传背景；在该研究所用品种中，贵州省油料研究所选育的品种遗传基础相对较广，但黔油17与黔油29的遗传相似性系数达0.87，遗传基础较为相近。
[목적]종분자수평상료해귀주성감람형유채주추품충적유전다양성。[방법]이용국가유채구역시험 DNA지문감정사용적40대 SSR인물대귀주성9개감람형유채주추품충진행다태성검측，병진행취류분석。[결과]시험공확증출조대191개，기중다태성조대143개，다태성비솔위75%。경취류분석，9개유채품충피분위 A、B2족，기중 A족포함7개품충，B족위귀주성유료연구소선육적2개품충。 A족재상사계수0.6434처우분위 A1화 A22개아족，A1아족위귀주성유채연구소선육적3개품충，A2아족위귀주성유료연구소선육4개품충。[결론]동일단위선육출적품충도유경위접근적유전배경；재해연구소용품충중，귀주성유료연구소선육적품충유전기출상대교엄，단검유17여검유29적유전상사성계수체0.87，유전기출교위상근。
[Objective] The aim was to investigate the genetic diversity of the main commercial varieties of Brassica napus in Guizhou Province at the molecular level. [Method] Nine main commercial rapeseed cultivars in Guizhou were detected by 40 pairs of SSR primers used in the national regional trial of B. napus, and then clus-tering analysis was carried out. [Result] A total of 191 bands were amplified, and 143 (accounting for 75%) of them showed polymorphism among the nine rapeseed cultivars. By cluster analysis, the nine cultivars were divided into two groups. Group A included seven varieties, and group B consisted of two cultivars from Guizhou In-stitute of Oil Crops. The cultivars in group A were further divided into sub-groups A1 and A2 at the similarity coefficient of 0.643 4. Sub-group A1 included three culti-vars from Guizhou Rapeseed Institute, and sub-group A2 included the other four cultivars from Guizhou Institute of Oil Crops. [Conclusion] The cultivars bred by the same institute had similar genetic background. The cultivars from Guizhou Institute of Oil Crops showed wider genetic basis. However, the genetic similarity coefficient between Qianyou 17 and Qianyou 29 was up to 0.87, suggesting that they shared closer genetic basis.