浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
2期
196-200
,共5页
糖尿病肾病%肾小球%病理形态%氧化应激
糖尿病腎病%腎小毬%病理形態%氧化應激
당뇨병신병%신소구%병리형태%양화응격
diabetic nephropathy%glomerular%morphologic%oxidative stress
[目的]研究清热益气通络方治疗大鼠糖尿病肾病的作用机制。[方法]采用单侧肾切除腹腔注射链脲佐菌素方法建立大鼠糖尿病肾病模型。将成模大鼠分为模型组、中药组和厄贝沙坦组，连续治疗12W。观察各组①肾组织超氧歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）含量；②肾小球横截面积（glomerular area, GA）、系膜区面积（mesangium area, MA）、MA/GA、肾小球基底膜厚度（glomerular basement membrane thickness, GBMT）、足突平均宽度（width of the podocyte foot processes, FPW）、足突融合率（foot process fusion rate, FPFR）。[结果]与正常组比，模型组大鼠肾组织MDA增高，SOD、CAT、GSH-Px降低（P<0.01）；GA、MA、FPW、FPFR及GBMT增高，MA/GA降低（P<0.01或P<0.05）。清热益气通络方能降低糖尿病大鼠肾组织MDA 及GA、MA、FPW 、FPFR 、GBMT（P<0.01或P<0.05），升高SOD、CAT水平（P<0.01或P<0.05）；其提高SOD、CAT，降低MDA及PFPR的作用优于厄贝沙坦（P<0.05）。[结论]清热益气通络方对DN具有治疗和保护作用，其机制可能与提高肾组织抗氧化物质，减轻DN大鼠肾脏氧化应激损伤，从而改善DN大鼠肾脏病理有一定关系。
[目的]研究清熱益氣通絡方治療大鼠糖尿病腎病的作用機製。[方法]採用單側腎切除腹腔註射鏈脲佐菌素方法建立大鼠糖尿病腎病模型。將成模大鼠分為模型組、中藥組和阨貝沙坦組，連續治療12W。觀察各組①腎組織超氧歧化酶（SOD）、過氧化氫酶（CAT）、穀胱甘肽過氧化物酶（GSH-Px）、丙二醛（MDA）含量；②腎小毬橫截麵積（glomerular area, GA）、繫膜區麵積（mesangium area, MA）、MA/GA、腎小毬基底膜厚度（glomerular basement membrane thickness, GBMT）、足突平均寬度（width of the podocyte foot processes, FPW）、足突融閤率（foot process fusion rate, FPFR）。[結果]與正常組比，模型組大鼠腎組織MDA增高，SOD、CAT、GSH-Px降低（P<0.01）；GA、MA、FPW、FPFR及GBMT增高，MA/GA降低（P<0.01或P<0.05）。清熱益氣通絡方能降低糖尿病大鼠腎組織MDA 及GA、MA、FPW 、FPFR 、GBMT（P<0.01或P<0.05），升高SOD、CAT水平（P<0.01或P<0.05）；其提高SOD、CAT，降低MDA及PFPR的作用優于阨貝沙坦（P<0.05）。[結論]清熱益氣通絡方對DN具有治療和保護作用，其機製可能與提高腎組織抗氧化物質，減輕DN大鼠腎髒氧化應激損傷，從而改善DN大鼠腎髒病理有一定關繫。
[목적]연구청열익기통락방치료대서당뇨병신병적작용궤제。[방법]채용단측신절제복강주사련뇨좌균소방법건립대서당뇨병신병모형。장성모대서분위모형조、중약조화액패사탄조，련속치료12W。관찰각조①신조직초양기화매（SOD）、과양화경매（CAT）、곡광감태과양화물매（GSH-Px）、병이철（MDA）함량；②신소구횡절면적（glomerular area, GA）、계막구면적（mesangium area, MA）、MA/GA、신소구기저막후도（glomerular basement membrane thickness, GBMT）、족돌평균관도（width of the podocyte foot processes, FPW）、족돌융합솔（foot process fusion rate, FPFR）。[결과]여정상조비，모형조대서신조직MDA증고，SOD、CAT、GSH-Px강저（P<0.01）；GA、MA、FPW、FPFR급GBMT증고，MA/GA강저（P<0.01혹P<0.05）。청열익기통락방능강저당뇨병대서신조직MDA 급GA、MA、FPW 、FPFR 、GBMT（P<0.01혹P<0.05），승고SOD、CAT수평（P<0.01혹P<0.05）；기제고SOD、CAT，강저MDA급PFPR적작용우우액패사탄（P<0.05）。[결론]청열익기통락방대DN구유치료화보호작용，기궤제가능여제고신조직항양화물질，감경DN대서신장양화응격손상，종이개선DN대서신장병리유일정관계。
[Objective]To investigate the mechanism of Qingre Yiqi Tongluo(QRYQTL) fang in treating the diabetic nephropathy. [Methods]60 Sprague-Dawleg rats were divided into normal control group(N), Diabetic nephropathy model group(M) (model rats were prepared by intraperitoneal injection with STZ after unilateral nephrectomy), then the model rats were assigned into model control group, QRYQTL fang treatment group(Q) and Irbesartan treatment group(I). After 12 weeks of the treatment, the contents of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px) and malondialdehyde (MDA) in renal tissue were examined by enzyme linked immunoassay(ELISA); the glomerular area(GA), mesangium area(MA),MA/GA, mean width of the podocyte foot processes(FPW) ,foot process fusion rate(FPFR) and average thickness of glomerular basement membrane(GBMT) were measured. [Results]Compared with N group, the contents of MDA in kidney homogenate and the GA,MA, FPW,FPFR,GBMT were markedly increased (P<0.01or P<0.05),the contents of SOD,CAT, GSH-Px and MA/GA were significantly decreased( P<0.01or P<0.05). QRYQTL fang reduced the level of MDA and decreased the GA, FPW, FPFR and GBMT(P<0.01or P<0.05). Compared with I group ,the QRYQTL fang was superior to Irbesartan in reducing the MDA, FPFR as wel as increasing the SOD,CAT(P<0.05).[ Conclusions]QRYQTL fang has a protective effect on the experimental diabec-tic nephropathy rats. The mechanism may be related to inhibiting oxidative stress, improving the body ’s antioxidant ability and al eviating the glomerular morphologic injury.
