CAJ | 학술논문

利用qRT-PCR系统分析了高温诱导对8月龄尼罗罗非鱼(Oreochromis niloticus)性腺组织中8个性别分化相关基因、4个热激蛋白基因及2个甲基转移酶基因表达的影响。结果显示，高温诱导能显著下调卵巢发育相关基因Cyp19a1a、ERα和ERβ的表达水平(P<0.05)，但对雌鱼精巢发育相关基因Cyp11b2和Dmrt1的表达水平没有显著影响(P>0.05)，这与高温对性腺分化期罗非鱼性别分化相关基因表达的影响不同。高温诱导后雌雄鱼性腺中Hsp70的表达水平显著上升(P<0.05)，而DNAJB1的表达水平却显著下降(P<0.05)， Hsp27和Hsp90的表达水平无显著变化。高温诱导能显著上调雌鱼性腺中Dnmt1与Dnmt3a基因的表达水平(P<0.05)。本研究通过分析高温诱导对性成熟尼罗罗非鱼性别分化相关基因表达的影响，旨在揭示高温诱导对罗非鱼性别分化相关基因表达的影响具有一定的时期特异性，为研究温度影响鱼类性别分化的分子机理提供基础。
이용qRT-PCR계통분석료고온유도대8월령니라라비어(Oreochromis niloticus)성선조직중8개성별분화상관기인、4개열격단백기인급2개갑기전이매기인표체적영향。결과현시，고온유도능현저하조란소발육상관기인Cyp19a1a、ERα화ERβ적표체수평(P<0.05)，단대자어정소발육상관기인Cyp11b2화Dmrt1적표체수평몰유현저영향(P>0.05)，저여고온대성선분화기라비어성별분화상관기인표체적영향불동。고온유도후자웅어성선중Hsp70적표체수평현저상승(P<0.05)，이DNAJB1적표체수평각현저하강(P<0.05)， Hsp27화Hsp90적표체수평무현저변화。고온유도능현저상조자어성선중Dnmt1여Dnmt3a기인적표체수평(P<0.05)。본연구통과분석고온유도대성성숙니라라비어성별분화상관기인표체적영향，지재게시고온유도대라비어성별분화상관기인표체적영향구유일정적시기특이성，위연구온도영향어류성별분화적분자궤리제공기출。
In Nile tilapia, the mRNA expression level of various sex differentiation-related genes can be affected by high-temperature induction during the critical period of sexual differentiation, triggering sex-reversal from female to physiological male. However, it is unknown whether the effect of high temperature on Nile tilapia (Oreochromis niloti-cus) sex differentiation-related genes is period-specific. In this study, the expression of eight sex differentiation-related genes, four heat shock protein genes and two methyltransferase genes were systematically analyzed in 8-month-old Nile tilapia under high-temperature (36℃) treatment by quantitative real-time RT-PCR techniques. The results showed that the expression levels of Cyp19a1a, ERα and ERβ, which are ovary development-related genes, were down-regulated (P<0.05), but the expression levels of Cyp11b2 and Dmrt1, which are testis development-related genes, were not sig-nificantly changed (P>0.05) under high-temperature treatment. The results differed from those for high-temperature treatment of Nile tilapia during the critical period of sexual differentiation. The expression level of Hsp70 was signifi-cantly up-regulated, while the expression level of DNAJB1 was significantly down-regulated. The expression levels of Hsp27and Hsp90 were not significantly changed in the gonads of females and males under high-temperature induction. Furthermore, it was also found that high temperature significantly up-regulated the expression level of Dnmt1 and Dnmt3a in the female gonad.