中国动物保健
中國動物保健
중국동물보건
CHINA ANIMAL HEALTH
2014年
2期
26-28
,共3页
余德照%姚荣英%周臣飞%季朝金
餘德照%姚榮英%週臣飛%季朝金
여덕조%요영영%주신비%계조금
杆菌肽锌预混剂%测定%锌含量%原子吸收
桿菌肽鋅預混劑%測定%鋅含量%原子吸收
간균태자예혼제%측정%자함량%원자흡수
bacitracin zinc premix%determination%zinc content%atomic absorption
本文阐述利用锌元素的特征谱线(锌原子从基态到激发态在213.8 nm处有最大吸收),创建了原子吸收法测定杆菌肽锌预混剂中锌的含量,并对方法进行了验证。结果表明,该方法在锌离子浓度为0.25~1.00μg/mL的范围内,浓度与吸光度之间具有良好的线性关系,回归方程为y=0.401x+0.0437相关系数r=0.9975,且精密度高、平均回收率为100.1% RSD=2.0%。
本文闡述利用鋅元素的特徵譜線(鋅原子從基態到激髮態在213.8 nm處有最大吸收),創建瞭原子吸收法測定桿菌肽鋅預混劑中鋅的含量,併對方法進行瞭驗證。結果錶明,該方法在鋅離子濃度為0.25~1.00μg/mL的範圍內,濃度與吸光度之間具有良好的線性關繫,迴歸方程為y=0.401x+0.0437相關繫數r=0.9975,且精密度高、平均迴收率為100.1% RSD=2.0%。
본문천술이용자원소적특정보선(자원자종기태도격발태재213.8 nm처유최대흡수),창건료원자흡수법측정간균태자예혼제중자적함량,병대방법진행료험증。결과표명,해방법재자리자농도위0.25~1.00μg/mL적범위내,농도여흡광도지간구유량호적선성관계,회귀방정위y=0.401x+0.0437상관계수r=0.9975,차정밀도고、평균회수솔위100.1% RSD=2.0%。
This paper presents the determination of zinc content in bacitracin zinc premix with atomic absorption (AAS) method. This method was established on the basis of characteristic spectral line of Zn (the maximum ab-sorption of Zn is obtained at 213.8 nm) and had been validated. It demonstrated that the good linearity between concentrations and absorptions was appeared when the range of concentrations is 0.25~1.00μg/mL;the corre-lation coefficient r was 0.9975 calculated with the formula y=0.401x+0.0437;precision was demonstrated and the recovery was 100.1%and the RSD was 2.0%.