CAJ | 학술논문

选择健康、均匀,体质量为(6.74±0.08) g的凡纳滨对虾(Litopenaeus vannamei),将360尾随机分为4组,分别投喂含有0 mg/kg、100 mg/kg、200 mg/kg和400 mg/kg绿原酸的饲料28 d,随后将对虾从盐度为32的天然海水直接转移至盐度为10的海水中暴露72 h,测定对虾肝胰腺TAS、GPx、CAT活力和GPx、CAT基因表达水平。结果显示,在天然海水养殖条件下,绿原酸对凡纳滨对虾肝胰腺TAS、GPx活性无明显影响,但投喂含有绿原酸的饲料28 d,对虾肝胰腺 GPx、CAT 和 GPx、CAT 基因表达水平显著高于 D0组(P<0.05),以 D2组 GPx 活性和 GPx、CAT基因表达水平最高,分别为164.29 U/mgprot和1.61、2.14倍。低盐度胁迫24 h, D0组对虾抗氧化反应表现在TAS、GPx活性和GPx基因表达水平较28 d显著升高(P<0.05),分别增加了31.30%、27.96%和170%,而绿原酸各组对虾肝胰腺TAS、GPx活性和GPx基因表达水平显著低于D0组(P<0.05),说明绿原酸可有效缓解低盐度胁迫下对虾抗氧化系统酶活性的剧烈波动。低盐度胁迫72 h,绿原酸各组对虾肝胰腺TAS、GPx、CAT活性及CAT基因表达水平均明显高于D0组。上述结果表明绿原酸能够诱导凡纳滨对虾的抗氧化系统功能,在凡纳滨对虾抵抗低盐度胁迫中发挥着重要的作用。本研究旨为开发绿色植物提取物在对虾养殖中的应用,解决盐度胁迫对机体造成的氧化损伤提供理论依据。
선택건강、균균,체질량위(6.74±0.08) g적범납빈대하(Litopenaeus vannamei),장360미수궤분위4조,분별투위함유0 mg/kg、100 mg/kg、200 mg/kg화400 mg/kg록원산적사료28 d,수후장대하종염도위32적천연해수직접전이지염도위10적해수중폭로72 h,측정대하간이선TAS、GPx、CAT활력화GPx、CAT기인표체수평。결과현시,재천연해수양식조건하,록원산대범납빈대하간이선TAS、GPx활성무명현영향,단투위함유록원산적사료28 d,대하간이선 GPx、CAT 화 GPx、CAT 기인표체수평현저고우 D0조(P<0.05),이 D2조 GPx 활성화 GPx、CAT기인표체수평최고,분별위164.29 U/mgprot화1.61、2.14배。저염도협박24 h, D0조대하항양화반응표현재TAS、GPx활성화GPx기인표체수평교28 d현저승고(P<0.05),분별증가료31.30%、27.96%화170%,이록원산각조대하간이선TAS、GPx활성화GPx기인표체수평현저저우D0조(P<0.05),설명록원산가유효완해저염도협박하대하항양화계통매활성적극렬파동。저염도협박72 h,록원산각조대하간이선TAS、GPx、CAT활성급CAT기인표체수평균명현고우D0조。상술결과표명록원산능구유도범납빈대하적항양화계통공능,재범납빈대하저항저염도협박중발휘착중요적작용。본연구지위개발록색식물제취물재대하양식중적응용,해결염도협박대궤체조성적양화손상제공이론의거。
To investigate the effects of chlorogenic acid on the antioxidant activity and resistance to low-salinity stress in Litopenaeus vannamei, 360 shrimps were randomly divided into four groups and fed four diets containing 0, 100, 200 and 400 mg chlorogenic acid/kg(diet). After feeding for 28 days, all shrimps were subjected to an acute salinity change from 32 to 10 for 72 h. Total antioxidant status (TAS), glutathione peroxidase (GPx) and catalase (CAT) levels, and ex-pression of the GPx and CAT genes in the hepatopancreas of L. vannamei, were analyzed. The results demonstrated that supplemental chlorogenic acid had no effect on TAS or on GPx activity in the hepatopancreas when shrimp were cul-tured in natural seawater. Compared with the D0 control group, however, dietary supplemental chlorogenic acid sig-nificantly increased GPx and CAT activities, and expression of the GPx and CAT gene in the hepatopancreas after feed-ing the shrimp for 28 days (P<0.05). GPx activity and gene expressions of GPx and CAT in the D2 group [200 mg (chlorogenic acid)/kg(diet) ] were higher than in the other groups, at 164.29 U/mg(prot) and 1.61 and 2.14 fold, respec-tively. After low-salinity stress for 24 h, the antioxidant response was characterized by higher TAS, greater GPx activity and higher gene expression of GPx in the hepatopancreas compared with levels found at 28 days (P<0.05), i.e. an in-crease of 31.30%, 27.96%and 170%, respectively. Moreover, TAS, GPx activity and GPx gene expression in the hepa-topancreas in shrimp that were fed diets supplemented with chlorogenic acid were lower than those of the D0 group (P<0.05). The results indicate that chlorogenic acid could effectively inhibit dramatic changes in antioxidant enzyme system activities of shrimp exposed to low-salinity stress. After low-salinity stress for 72 h, TAS, GPx and CAT activi-ties and GPx gene expression in the hepatopancreas of shrimp that were fed chlorogenic acid were higher than the D0 group. In conclusion, chlorogenic acid induces antioxidant system activity and plays an important role in resistance to low-salinity stress in L. vannamei.