CAJ | 학술논문

在凡纳滨对虾(Litopenaeus vannamei)零换水室内水泥池高密度养殖系统中，使用生物填料进行微生物固定化，并通过泼洒碳酸氢钠(NaHCO3)，将养殖池的碱度分别控制在： T1处理组130 mg (CaCO3)/L； T2处理组100 mg (CaCO3)/L；T3处理组70 mg (CaCO3)/L；T4处理组不调节，每处理组设置3个重复。在养殖周期内定期检测各水层和各生物填料层微生物的数量变化，以及水质参数和对虾生长性状参数，结果表明， T1和T2处理组在各水层和填料层的细菌总数和氮循环细菌数量，显著高于 T3和 T4处理组(P＜0.05)，总氮浓度、无机氮浓度、对虾体质量增长速度、饵料转化率等参数亦显著优于T3和T4处理组(P＜0.05)。将碱度提高到100 mg (CaCO3)/L以上，可有效提高高密度养殖系统中固定化微生物的处理效果，从而提高养殖效益。
재범납빈대하(Litopenaeus vannamei)령환수실내수니지고밀도양식계통중，사용생물전료진행미생물고정화，병통과발쇄탄산경납(NaHCO3)，장양식지적감도분별공제재： T1처리조130 mg (CaCO3)/L； T2처리조100 mg (CaCO3)/L；T3처리조70 mg (CaCO3)/L；T4처리조불조절，매처리조설치3개중복。재양식주기내정기검측각수층화각생물전료층미생물적수량변화，이급수질삼수화대하생장성상삼수，결과표명， T1화T2처리조재각수층화전료층적세균총수화담순배세균수량，현저고우 T3화 T4처리조(P＜0.05)，총담농도、무궤담농도、대하체질량증장속도、이료전화솔등삼수역현저우우T3화T4처리조(P＜0.05)。장감도제고도100 mg (CaCO3)/L이상，가유효제고고밀도양식계통중고정화미생물적처리효과，종이제고양식효익。
Alkalinity control in an indoor, concrete pond, zero water exchange high-density culture system for Litope-naeus vannamei, using bio-filler microbial immobilization and addition of sodium bicarbonate (NaHCO3), was tested at various levels. Level T1 involved treatment with 130 mg(CaCO3)/L, T2 was treated with 100 mg(CaCO3)/L, T3 was treated with 70 mg(CaCO3)/L, and the T4 treatment group was not adjusted and acted as the control. There were three replicates for each treatment group. The number of microorganisms in the water and biological filler layers were re-corded regularly during the breeding cycle, and water quality parameters and shrimp growth traits parameters were monitored. The results show that the T1 and T2 treatments had a significantly better effect on the total number of bacte-ria in the water and biological filler layers plus the nitrogen cycle. In T1 and T2, the number of bacteria was signifi-cantly higher than in the T3 and T4 treatment groups (P<0.05), while the concentration of total nitrogen and inorganic nitrogen, shrimp weight gain, feed conversion rate, and other parameters were also significantly better than in the T3 and T4 treatment group (P<0.05). The higher the alkalinity was maintained, the shorter the time interval of adjustment in adding sodium bicarbonate. In the late breeding period, the high alkalinity of the water and filler layer and thus total number of bacteria and nitrogen cycle bacteria were significantly higher than the number in low-alkalinity water. This trend was more obvious with increasing water depth. The mechanism of the ecological impact of alkalinity in shrimp farming mainly affects the number of microbes and microbial decomposition efficiency. The growth of microorganisms and increase in decomposition efficiency reduces ammonia nitrogen and inorganic nitrogen concentrations, and thereby maintains a better growth environment for shrimp and thus the growth of shrimp. In conclusion, in a high-density shrimp farming system, raising the alkalinity to 100 mg(CaCO3)/L or more can effectively increase the number of im-mobilized microorganisms, thereby improving breeding efficiency. As regards reducing the amount of alkalin-ity-adjusting agent and ease of management, maintaining water alkalinity at 100 mg(CaCO3)/L is appropriate.