中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
4期
733-737
,共5页
川崎病%细胞免疫%T-bet蛋白%GATA3转录因子
川崎病%細胞免疫%T-bet蛋白%GATA3轉錄因子
천기병%세포면역%T-bet단백%GATA3전록인자
Kawasaki disease%Cellular immunity%T-bet protein%GATA3 transcription factor
目的：研究川崎病患儿细胞免疫功能状况及转录因子T-bet和GATA3的调控作用。方法：选取41例川崎病患儿为研究对象，同时选取年龄和性别相匹配的因急性上呼吸道感染发热儿童40例为对照组，分别采用三色荧光流式细胞术和半定量逆转录-聚合酶链反应检测2组患儿外周血Th1、Th2细胞数量及单个核细胞中T-bet和GATA3 mRNA水平。结果：川崎病患儿外周血Th1、Th2细胞比率及单个核细胞转录因子T-bet mRNA、GA-TA3 mRNA转录水平分别为（10．36±3．69）％、（6．46±2．28）％、0．51±0．16和0．38±0．13，均显著低于对照组[（25．26±5．22）％、（16．87±4．35）％、0．62±0．21和0．46±0．12，P＜0．05]。 Spearman相关分析显示Th1和Th2细胞比率分别与T-bet mRNA和GATA3 mRNA水平呈正相关。结论：川崎病患儿急性期T细胞并非活化而是处于抑制状态，T-bet和GATA3分别对Th1和Th2细胞增殖具有调控作用。
目的：研究川崎病患兒細胞免疫功能狀況及轉錄因子T-bet和GATA3的調控作用。方法：選取41例川崎病患兒為研究對象，同時選取年齡和性彆相匹配的因急性上呼吸道感染髮熱兒童40例為對照組，分彆採用三色熒光流式細胞術和半定量逆轉錄-聚閤酶鏈反應檢測2組患兒外週血Th1、Th2細胞數量及單箇覈細胞中T-bet和GATA3 mRNA水平。結果：川崎病患兒外週血Th1、Th2細胞比率及單箇覈細胞轉錄因子T-bet mRNA、GA-TA3 mRNA轉錄水平分彆為（10．36±3．69）％、（6．46±2．28）％、0．51±0．16和0．38±0．13，均顯著低于對照組[（25．26±5．22）％、（16．87±4．35）％、0．62±0．21和0．46±0．12，P＜0．05]。 Spearman相關分析顯示Th1和Th2細胞比率分彆與T-bet mRNA和GATA3 mRNA水平呈正相關。結論：川崎病患兒急性期T細胞併非活化而是處于抑製狀態，T-bet和GATA3分彆對Th1和Th2細胞增殖具有調控作用。
목적：연구천기병환인세포면역공능상황급전록인자T-bet화GATA3적조공작용。방법：선취41례천기병환인위연구대상，동시선취년령화성별상필배적인급성상호흡도감염발열인동40례위대조조，분별채용삼색형광류식세포술화반정량역전록-취합매련반응검측2조환인외주혈Th1、Th2세포수량급단개핵세포중T-bet화GATA3 mRNA수평。결과：천기병환인외주혈Th1、Th2세포비솔급단개핵세포전록인자T-bet mRNA、GA-TA3 mRNA전록수평분별위（10．36±3．69）％、（6．46±2．28）％、0．51±0．16화0．38±0．13，균현저저우대조조[（25．26±5．22）％、（16．87±4．35）％、0．62±0．21화0．46±0．12，P＜0．05]。 Spearman상관분석현시Th1화Th2세포비솔분별여T-bet mRNA화GATA3 mRNA수평정정상관。결론：천기병환인급성기T세포병비활화이시처우억제상태，T-bet화GATA3분별대Th1화Th2세포증식구유조공작용。
AIM:To clarify the functional state of T cells and the role of transcription factors T-bet and GA-TA3 in choldren with Kawasaki disease .METHODS:The patients with acute Kawasaki disease ( n=41) were enrolled as experimental group and 40 febrile children with acute upper respiratory tract infection were selected as control group .The mRNA expression levels of T-bet and GATA3, and the percentages of Th1 and Th2 cells were examined by RT-PCR and flow cytometry respectively .RESULTS:The mRNA expression levels of T-bet and GATA3 and the percentages of Th 1 and Th2 cells in Kawasaki disease group were 0.51 ±0.16, 0.38 ±0.13, (10.36 ±3.69)%and (6.46 ±2.28)%, respec-tively, and were significantly lower than those in control group [0.62 ±0.21, 0.46 ±0.12, (25.26 ±5.22)% and (16.87 ±4.35)%, P<0.05].There were positive correlations between the mRNA expression levels of T-bet and GA-TA3, and the percentages of Th1 and Th2 cells.CONCLUSION:Associated with the suppression of both T-bet and GA-TA3 mRNA expression, the function of Th1 and Th2 cells are suppressed simultaneously in acute Kawasaki disease .
