CAJ | 학술논문

目的：探讨金雀异黄素（Gen）对氧化损伤的人晶状体上皮细胞（HLEC）凋亡有无抑制作用。方法：本研究在终浓度3×10－4mol/L H2O2培养液中复制HLEC凋亡模型，并以雌二醇（E2）作为阳性对照，采用流式细胞仪（FCM）检测HLEC凋亡率和线粒体膜电位（ΔΨM）的变化。结果：FCM结果显示：H2 O2组HLEC凋亡率显著高于空白对照组；E2和Gen组凋亡率均低于H2O2组（P＜0．01）。 H2O2组线粒体膜电位比对照组显著下降；E2组和Gen组的线粒体膜电位均比H2O2组升高（P＜0．01）。结论：Gen可减轻实验性氧化损伤的HLEC凋亡程度。
목적：탐토금작이황소（Gen）대양화손상적인정상체상피세포（HLEC）조망유무억제작용。방법：본연구재종농도3×10－4mol/L H2O2배양액중복제HLEC조망모형，병이자이순（E2）작위양성대조，채용류식세포의（FCM）검측HLEC조망솔화선립체막전위（ΔΨM）적변화。결과：FCM결과현시：H2 O2조HLEC조망솔현저고우공백대조조；E2화Gen조조망솔균저우H2O2조（P＜0．01）。 H2O2조선립체막전위비대조조현저하강；E2조화Gen조적선립체막전위균비H2O2조승고（P＜0．01）。결론：Gen가감경실험성양화손상적HLEC조망정도。
Objective:To investigate if Genistein(Gen) inhibits the apoptosis of human lens epithelial cell (HLEC) resulted from the ex-perimental oxidative injury .Methods:HLECs were incubated with 3 ×10 -4 mol/L H2 O2 .And make estradiol ( E2 ) as a positive con-trol.HLECs apoptosis and apoptosis rate were measured by FCM .Results:Apoptosis rate in H2 O2 group was significandy higher than that in control group , E2 and Gen group .The mitochondria transmembrane potential in H 2 O2 group reduced obviously , comparing to control group, E2 and Gen group.Conclusion:Gen may decrease the apoptosis of HLEC resulted from the experimental oxidative inju-ry, providing scientific evidence for pursue effective medicine to prevent and treat cataract .