江西中医学院学报
江西中醫學院學報
강서중의학원학보
JOURNAL OF JIANGXI COLLEGE OF TRADITIONAL CHINESE MEDICINE
2014年
1期
62-63,64
,共3页
涂秀英%李瑛%魏学鑫%于梅%徐国良%章常华
塗秀英%李瑛%魏學鑫%于梅%徐國良%章常華
도수영%리영%위학흠%우매%서국량%장상화
小檗碱%黄芩苷%葡萄糖氧化酶法%干扰作用
小檗堿%黃芩苷%葡萄糖氧化酶法%榦擾作用
소벽감%황금감%포도당양화매법%간우작용
Berberine%Baicalin%Glucose Oxidase Assay%Interference Effect
目的:探讨小檗碱和黄芩苷对葡萄糖氧化酶( GOD)法测定葡萄糖含量的干扰作用。方法:用GOD法测定加入不同浓度小檗碱和黄芩苷的含血清培养基葡萄糖含量结果,对其结果进行分析、比较。结果:对照组与小檗碱和黄芩苷给药组间葡萄糖含量无显著差异,表明小檗碱和黄芩苷对GOD测定葡萄糖含量无明显干扰作用(1~200μmol/L),结论:在用GOD法测定葡萄糖含量时,小檗碱和黄芩苷对GOD测定葡萄糖含量无明显干扰作用(1~200μmol/L)。
目的:探討小檗堿和黃芩苷對葡萄糖氧化酶( GOD)法測定葡萄糖含量的榦擾作用。方法:用GOD法測定加入不同濃度小檗堿和黃芩苷的含血清培養基葡萄糖含量結果,對其結果進行分析、比較。結果:對照組與小檗堿和黃芩苷給藥組間葡萄糖含量無顯著差異,錶明小檗堿和黃芩苷對GOD測定葡萄糖含量無明顯榦擾作用(1~200μmol/L),結論:在用GOD法測定葡萄糖含量時,小檗堿和黃芩苷對GOD測定葡萄糖含量無明顯榦擾作用(1~200μmol/L)。
목적:탐토소벽감화황금감대포도당양화매( GOD)법측정포도당함량적간우작용。방법:용GOD법측정가입불동농도소벽감화황금감적함혈청배양기포도당함량결과,대기결과진행분석、비교。결과:대조조여소벽감화황금감급약조간포도당함량무현저차이,표명소벽감화황금감대GOD측정포도당함량무명현간우작용(1~200μmol/L),결론:재용GOD법측정포도당함량시,소벽감화황금감대GOD측정포도당함량무명현간우작용(1~200μmol/L)。
Objective:To investigate the interference effect of berberine and baicalin on glucose oxidase assay in determination of the content of glucose .Methods:To determine glucose concentration of medium containing different concentrations of berberine and baica -lin by the method of glucose oxidase .Results: There was no significant change between the berberine group and the control ( P>0.05), indicating that berberine and baicalin themselves at the concentration (1~200 μmol/L) used in the experiment do not cause interference.Conclusion: The experimental results indicated that berberine and baicalin themselves at the concentration (1 ~200μmol/L) used in the experiment do not cause interference .