吉林林业科技
吉林林業科技
길림임업과기
JILIN FORESTRY SCICNCE AND TECHNOLOGY
2014年
2期
7-8,59
,共3页
百日草%组织培养%无性繁殖
百日草%組織培養%無性繁殖
백일초%조직배양%무성번식
Zinnia elegans%tissue culture%vegetative propagation
以百日草茎段为外植体，对其进行组织培养技术研究，结果表明：不同生长阶段的茎段对相同的消毒处理产生不同的反映，以生长中等的茎段诱导腋芽萌发的效果最为理想，适宜的灭菌方法为70％酒精处理20 s、再用0.1％HgCl2处理8＋4 min。最合适的腋芽诱导培养基为MS＋KT0．2 mg· L-1＋BA2．0 mg· L-1＋GA0．5 mg· L-1，外植体污染率较低，诱导率较高；试管苗继代增殖效果最佳的培养基为MS＋BA1．0 mg· L-1，不仅增殖系数高，达到8．67，而且试管苗生长健壮；生根效果最好的培养基为 MS＋NAA0．1 mg · L-1，试管苗根系生长粗壮，生根数量多，平均根长较短。
以百日草莖段為外植體，對其進行組織培養技術研究，結果錶明：不同生長階段的莖段對相同的消毒處理產生不同的反映，以生長中等的莖段誘導腋芽萌髮的效果最為理想，適宜的滅菌方法為70％酒精處理20 s、再用0.1％HgCl2處理8＋4 min。最閤適的腋芽誘導培養基為MS＋KT0．2 mg· L-1＋BA2．0 mg· L-1＋GA0．5 mg· L-1，外植體汙染率較低，誘導率較高；試管苗繼代增殖效果最佳的培養基為MS＋BA1．0 mg· L-1，不僅增殖繫數高，達到8．67，而且試管苗生長健壯；生根效果最好的培養基為 MS＋NAA0．1 mg · L-1，試管苗根繫生長粗壯，生根數量多，平均根長較短。
이백일초경단위외식체，대기진행조직배양기술연구，결과표명：불동생장계단적경단대상동적소독처리산생불동적반영，이생장중등적경단유도액아맹발적효과최위이상，괄의적멸균방법위70％주정처리20 s、재용0.1％HgCl2처리8＋4 min。최합괄적액아유도배양기위MS＋KT0．2 mg· L-1＋BA2．0 mg· L-1＋GA0．5 mg· L-1，외식체오염솔교저，유도솔교고；시관묘계대증식효과최가적배양기위MS＋BA1．0 mg· L-1，불부증식계수고，체도8．67，이차시관묘생장건장；생근효과최호적배양기위 MS＋NAA0．1 mg · L-1，시관묘근계생장조장，생근수량다，평균근장교단。
Stems of Zinnia elegans were taken as explants to research on the tissue culture technique; the results showed that the responses of different growth stages of stems for the same disinfection treatment were different .Medium stem had the most ideal effect of axillary bud sprouting , the suitable sterilization method was with 70%alcohol for 20 s, and then re-used with 0.1%HgCl for 8+4 minutes.MS+KT0.2 mg· L-1 +BA2.0 mg· L-1 +GA0.5 mg· L-1 was the most appro-priate medium of axillary bud induction , with low pollution rate of explant and high induction rate;MS+BA1.0 mg· L-1 was the most appropriate medium , which had the best multiplication effect of subculture of test -tube plantlet ;growth co-efficient reached 8.67, and the test-tube plantlet grew strongly;MS+NAA0.1 mg mg· L-1 was the most appropriate me-dium of rooting effect , the roots of test-tube plantlet grew strongly , the average root length was shorter , and quantity was large.
