南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2014年
3期
391-395
,共5页
近红外线拉曼光谱%胃癌%胃癌前病变%光学诊断
近紅外線拉曼光譜%胃癌%胃癌前病變%光學診斷
근홍외선랍만광보%위암%위암전병변%광학진단
near-infrared Raman spectroscopy%gastric cancer%gastric precancerous lesions%optical diagnosis
目的：采用近红外线激光拉曼光谱技术研究胃癌组织、胃癌前病变组织及正常胃粘膜组织的拉曼光谱的差异变化，建立一种胃癌光学诊断的方法。方法收集病理学证实的33例胃癌组织、27例胃癌前病变组织及45例正常胃粘膜组织，用近红外线激光拉曼光谱仪进行检测胃病变组织拉曼峰强度比值，所有标本来自105例受试者均为接受胃切除或是接受胃镜下对胃部可疑病灶进行活检的患者。结果④典型的拉曼谱峰位于853 cm-1，936 cm-1，1003 cm-1，1032 cm-1，1174 cm-1，1208 cm-1，1323 cm-1，1335 cm-1，1450 cm-1，1655 cm-1谱带处，胃癌组、胃癌前病变组及正常组的激光拉曼光谱分布存在差异（P<0.05，单向方差分析，95%可信区间）；④拉曼峰强度比值比较：I1003/I1337，I1003/I1450，I1003/I1655，I156/I1655，健康对照组显著高于胃癌组及胃癌前病变组，三者两两比较差异有统计学意义（P<0.05，单向方差分析，95%可信区间）；④采用PCA-LDA诊断算法得到正常胃组织、胃癌前病变组织及胃癌组织的灵敏度分别是：81.5%，85.3%，100%，特异度分别是：86%，100%，97.4%。结论近红外线激光拉曼光谱分析技术联合拉曼峰强度比值具有在分子水平诊断胃癌的价值。
目的：採用近紅外線激光拉曼光譜技術研究胃癌組織、胃癌前病變組織及正常胃粘膜組織的拉曼光譜的差異變化，建立一種胃癌光學診斷的方法。方法收集病理學證實的33例胃癌組織、27例胃癌前病變組織及45例正常胃粘膜組織，用近紅外線激光拉曼光譜儀進行檢測胃病變組織拉曼峰彊度比值，所有標本來自105例受試者均為接受胃切除或是接受胃鏡下對胃部可疑病竈進行活檢的患者。結果④典型的拉曼譜峰位于853 cm-1，936 cm-1，1003 cm-1，1032 cm-1，1174 cm-1，1208 cm-1，1323 cm-1，1335 cm-1，1450 cm-1，1655 cm-1譜帶處，胃癌組、胃癌前病變組及正常組的激光拉曼光譜分佈存在差異（P<0.05，單嚮方差分析，95%可信區間）；④拉曼峰彊度比值比較：I1003/I1337，I1003/I1450，I1003/I1655，I156/I1655，健康對照組顯著高于胃癌組及胃癌前病變組，三者兩兩比較差異有統計學意義（P<0.05，單嚮方差分析，95%可信區間）；④採用PCA-LDA診斷算法得到正常胃組織、胃癌前病變組織及胃癌組織的靈敏度分彆是：81.5%，85.3%，100%，特異度分彆是：86%，100%，97.4%。結論近紅外線激光拉曼光譜分析技術聯閤拉曼峰彊度比值具有在分子水平診斷胃癌的價值。
목적：채용근홍외선격광랍만광보기술연구위암조직、위암전병변조직급정상위점막조직적랍만광보적차이변화，건립일충위암광학진단적방법。방법수집병이학증실적33례위암조직、27례위암전병변조직급45례정상위점막조직，용근홍외선격광랍만광보의진행검측위병변조직랍만봉강도비치，소유표본래자105례수시자균위접수위절제혹시접수위경하대위부가의병조진행활검적환자。결과④전형적랍만보봉위우853 cm-1，936 cm-1，1003 cm-1，1032 cm-1，1174 cm-1，1208 cm-1，1323 cm-1，1335 cm-1，1450 cm-1，1655 cm-1보대처，위암조、위암전병변조급정상조적격광랍만광보분포존재차이（P<0.05，단향방차분석，95%가신구간）；④랍만봉강도비치비교：I1003/I1337，I1003/I1450，I1003/I1655，I156/I1655，건강대조조현저고우위암조급위암전병변조，삼자량량비교차이유통계학의의（P<0.05，단향방차분석，95%가신구간）；④채용PCA-LDA진단산법득도정상위조직、위암전병변조직급위암조직적령민도분별시：81.5%，85.3%，100%，특이도분별시：86%，100%，97.4%。결론근홍외선격광랍만광보분석기술연합랍만봉강도비치구유재분자수평진단위암적개치。
Objective To establish a method for early diagnosis of gastric cancer using near-infrared Raman spectroscopy. Methods A rapid near-infrared Raman system was used to examine the tissue specimens of pathologically confirmed gastric cancer (33 cases), gastric precancerous lesions (27 cases), and normal gastric mucosa (45 cases). All the specimens were obtained from 105 patients undergoing gastrectomy or endoscopic biopsy of suspected gastric lesions. Results High-quality Raman spectra ranging from 700 to 1800 cm-1 were acquired from the gastric tissues within 5 s. The distribution pattern of Raman spectra in gastric cancer differed significantly from those of gastric precancerous lesions and normal gastric mucosa, particularly in the spectral ranges of 853 cm-1, 936 cm-1, 1003 cm-1, 1032 cm-1, 1174 cm-1, 1208 cm-1, 1323 cm-1, 1335 cm-1, 1450 cm-1, and 1655 cm-1, which contained signals related to proteins, nucleic acids and lipids. The diagnostic decision algorithm based on the Raman peak intensity ratios of I10 3/I13 7, I10 3/I14 5, I10 3/I165 , and I1 56/I165 yielded remarkable differences in gastric cancer from gastric precancerous lesions and normal gastric mucosa, and the ratios were significantly higher in normal gastric tissues (P<0.05). The discrimination based on near-infrared Raman spectroscopy using PCA-LDA algorithms associated with leave-one-out and cross-validation method showed diagnostic sensitivities of 81.5%, 85.3%, and 100%, and specificities of 86.4%, 100%, and 97.4%for normal gastric mucosa, precancerous lesions and gastric cancer, respectively. Conclusion near-infrared Raman spectroscopy in conjunction with intensity ratio algorithms shows the potential for noninvasive diagnosis and detection of gastric malignancy at the molecular level.
