CAJ | 학술논문

目的：研究盐酸椒苯酮胺（PPTA）对庆大霉素耳蜗损伤的保护作用及抗凋亡机制。方法听力正常豚鼠分3组：正常组、GM组和PPTA组。采用庆大霉素致豚鼠耳蜗损伤模型，PPTA腹腔注射，ABR分析听力变化，Western blot检测耳蜗组织中caspase-3蛋白表达，TUNEL染色、扫描电镜和透射电镜观察形态学改变。结果 ABR反应阈：GM组、PPTA组显著高于正常对照组（P<0.05），PPTA组显著低于GM组；Western blot测caspase-3的表达：结果表明用药后GM组豚鼠caspase-3表达显著增加（P<0.001），PPTA组显著高于正常组但显著低于GM组（P<0.001）；TUNEL、扫描和透射电镜观察见：GM组毛细胞损伤严重，耳蜗螺旋器、血管纹和螺旋神经节存在大量凋亡细胞形态；而PPTA组耳蜗组织损伤较GM组明显减轻。结论 PPTA可以通过降低caspase-3蛋白表达抑制凋亡，对庆大霉素豚鼠耳蜗损伤起到保护作用。
목적：연구염산초분동알（PPTA）대경대매소이와손상적보호작용급항조망궤제。방법은력정상돈서분3조：정상조、GM조화PPTA조。채용경대매소치돈서이와손상모형，PPTA복강주사，ABR분석은력변화，Western blot검측이와조직중caspase-3단백표체，TUNEL염색、소묘전경화투사전경관찰형태학개변。결과 ABR반응역：GM조、PPTA조현저고우정상대조조（P<0.05），PPTA조현저저우GM조；Western blot측caspase-3적표체：결과표명용약후GM조돈서caspase-3표체현저증가（P<0.001），PPTA조현저고우정상조단현저저우GM조（P<0.001）；TUNEL、소묘화투사전경관찰견：GM조모세포손상엄중，이와라선기、혈관문화라선신경절존재대량조망세포형태；이PPTA조이와조직손상교GM조명현감경。결론 PPTA가이통과강저caspase-3단백표체억제조망，대경대매소돈서이와손상기도보호작용。
Objective To study the protective effect of peperphentonamine hydrochloride (PPTA) against gentamicin-induced cochlear damage and its mechanism to inhibit cell apoptosis. Methods Guinea pigs with normal hearing were randomized into control, gentamicin, and PPTA treatment groups, and the guinea pigs models of gentamicin-induced cochlear damage received intraperitoneal injection of PPTA. The changes of hearing of the guinea pigs were evaluated with auditory brainstem response (ABR) test, and the protein expression of caspase-3 in the cochlear tissue was detected using Western blotting. TUNEL staining, scanning and transmission electron microscopy were performed to observe the morphological changes of the cochlea. Results The threshold in ABR in PPTA treatment group was significantly higher than that in the control group (P<0.05) but significantly lower than that in gentamicin group. Western blotting showed a significantly increased caspase-3 expression in gentamicin group (P<0.001); caspase-3 expression in PPTA group was obviously higher than that in the control group but much lower than that in gentamicin group (P<0.001). TUNEL assay and electron microscopy revealed serious damages of the hair cells in gentamicin group with numerous apoptotic cells in the organ of Corti, stria vascularis and spiral ganglion, and such cochlear damages were obviously alleviated in PPTA group. Conclusion PPTA can protect against gentamicin-induced cochlear damage in guinea pigs by decreasing the protein expression of caspase-3 to inhibit cell apoptosis.