CAJ | 학술논문

目的：通过对比观察AD模型小鼠海马与小脑Aβ沉积和相关miRNAs表达的变化，探讨AD模型小鼠小脑是否存在Aβ沉积和相关miRNAs的差异表达。方法选择12月龄大小的APPswe/PS△E9双转基因AD小鼠作为实验组（EG），同月龄的同种系的野生型小鼠C57为对照组（CG），每组各12只。将小鼠脑组织分为左右两部分，右侧脑组织行刚果红染色检测淀粉样物质在海马及小脑的沉积；左侧脑组织分别用于提取海马及小脑的miRNA，应用实时荧光定量PCR方法分别检测两组小鼠海马及小脑组织的miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p和miR-669f-3p的表达情况。结果刚果红染色：实验组小鼠海马及小脑均可见橘红色的Aβ沉积，对照组小鼠海马及小脑均未见橘红色的Aβ沉积。实时荧光定量PCR：四种miRNAs在实验组海马的表达均低于对照组（P<0.05）；miRNA-135a-5p、miRNA-298-5p和miR-669f-3p在实验组小脑的表达低于对照组（P<0.05）；miRNA-298-5p和miR-669f-3p在实验组海马的表达低于小脑（P<0.05）。结论APPswe/PS△E9双转基因AD小鼠小脑中也存在Aβ沉积，其形成可能与miRNA-135a-5p、miRNA-298-5p和miR-669f-3p的表达下调有关。
목적：통과대비관찰AD모형소서해마여소뇌Aβ침적화상관miRNAs표체적변화，탐토AD모형소서소뇌시부존재Aβ침적화상관miRNAs적차이표체。방법선택12월령대소적APPswe/PS△E9쌍전기인AD소서작위실험조（EG），동월령적동충계적야생형소서C57위대조조（CG），매조각12지。장소서뇌조직분위좌우량부분，우측뇌조직행강과홍염색검측정분양물질재해마급소뇌적침적；좌측뇌조직분별용우제취해마급소뇌적miRNA，응용실시형광정량PCR방법분별검측량조소서해마급소뇌조직적miRNA-135a-5p、miRNA-298-5p、miRNA-466b-3p화miR-669f-3p적표체정황。결과강과홍염색：실험조소서해마급소뇌균가견귤홍색적Aβ침적，대조조소서해마급소뇌균미견귤홍색적Aβ침적。실시형광정량PCR：사충miRNAs재실험조해마적표체균저우대조조（P<0.05）；miRNA-135a-5p、miRNA-298-5p화miR-669f-3p재실험조소뇌적표체저우대조조（P<0.05）；miRNA-298-5p화miR-669f-3p재실험조해마적표체저우소뇌（P<0.05）。결론APPswe/PS△E9쌍전기인AD소서소뇌중야존재Aβ침적，기형성가능여miRNA-135a-5p、miRNA-298-5p화miR-669f-3p적표체하조유관。
Objective To investigate the presence of β-amyloid peptide (Aβ) deposition in the cerebellum and the expression of related miRNAs in the cerebellum of a mouse model of Alzheimer disease. Methods Twelve 12-month-old APPswe/PS△E9 double transgenic mice and 12 wild-type C57 mice were sacrificed and the brain tissues were taken for examination. The right hemisphere was stained with Congo red to observe the deposition of amyloid substances, and from the left hemisphere, the hippocampus and the cerebellum were dissected for detecting the expression of miRNA-135a-5p, miRNA-298-5p, miRNA-466b-3p and miR-669f-3p using real-time PCR. Results Congo red staining revealed the presence of Aβdeposition in both the hippocampus and the cerebellum of the transgenic mice but not in the control mice. Real-time PCR showed a significantly lower expression of the 4 miRNAs in the hippocampus in the transgenic mice than in the control mice (P<0.05). The expression of miRNA-135a-5p, miRNA-298-5p, and miR-669f-3p in the cerebellum was significantly lower in the transgenic mice than in the control mice (P<0.05). The expression of miRNA-298-5p and miR-669f-3p in the hippocampus was significantly lower than that in the cerebellum of the transgenic mice (P<0.05). Conclusion Aβdeposition also occurs in the cerebellum of APPswe/PS△E9 double transgenic mice, and its formation might be related to the down-regulation of miRNA-135a-5p, miRNA-298-5p, and miR-669f-3p.