CAJ | 학술논문

目的：联合血气、血液生化及组织形态变化探讨在冬季急进不同海拔（2500 m以内）环境下对大鼠心肌的影响。方法实验Wistar雄性大鼠30只，分为A组（海拔55 m）、B组（海拔1520 m）及C组（海拔2260 m）三组。正常饮水喂食，在各海拔处标准饲养3 d，动脉血测定血气，静脉血做生化检测，取心肌组织用比色法测定丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO），用细胞化学HE染色法及电镜观察左心室病理结构的改变。结果各组血气pH、PCO2均无差异，但C组均较A组PO2及BE明显下降（P<0.05）；心肌酶谱、MYO及Tn-I值C组较A组明显增加（P<0.01）；与A组相比C组心肌组织氧化应激的MDA明显增加（P<0.01），SOD明显下降（P<0.05），B、C组NO均较A组明显减少（P<0.01）；病理切片结果显示C组大鼠心肌细胞排列紊乱，肌纤维溶解断裂；电镜显示C组有部分肌丝断裂，少量线粒体扩张。结论在冬季急进低海拔2260 m处大鼠心肌有一定程度的损伤，其为高原低氧的氧化应激及环境变化动物应激所造成的综合结果。
목적：연합혈기、혈액생화급조직형태변화탐토재동계급진불동해발（2500 m이내）배경하대대서심기적영향。방법실험Wistar웅성대서30지，분위A조（해발55 m）、B조（해발1520 m）급C조（해발2260 m）삼조。정상음수위식，재각해발처표준사양3 d，동맥혈측정혈기，정맥혈주생화검측，취심기조직용비색법측정병이철（MDA）、초양화물기화매（SOD）、일양화담（NO），용세포화학HE염색법급전경관찰좌심실병리결구적개변。결과각조혈기pH、PCO2균무차이，단C조균교A조PO2급BE명현하강（P<0.05）；심기매보、MYO급Tn-I치C조교A조명현증가（P<0.01）；여A조상비C조심기조직양화응격적MDA명현증가（P<0.01），SOD명현하강（P<0.05），B、C조NO균교A조명현감소（P<0.01）；병리절편결과현시C조대서심기세포배렬문란，기섬유용해단렬；전경현시C조유부분기사단렬，소량선립체확장。결론재동계급진저해발2260 m처대서심기유일정정도적손상，기위고원저양적양화응격급배경변화동물응격소조성적종합결과。
Objective To investigate the influence of a sudden increase of altitudes (within 2500 m) in winter on cardiomyocyte functions in rats. Methods Thirty male Wistar rats were randomly divided into groups A (55 m), B (1520 m), and C (2260 m) and subjected to simulated conditions at the indicated altitudes in winter for 3 days. Blood gas analysis, venous blood biochemistry, and measurements of SOD activity and myocardial concentrations of MDA and NO were performed. Histopathological changes in the left ventricle were observed with HE staining and electron microscopy. Results Blood pH and PCO2 did not differ significantly between the 3 groups, but PO2 and BE in groups B and C decreased significantly compared with those in group A (P<0.01). Compared with group A, the rats in group C showed obviously increased myocardial enzymes, MYB, Tn-I, and MDA contents (P<0.01) with significantly decreased SOD activity (P<0.05); both groups B and C showed significantly decreased NO content in the myocardium (P<0.01). Histopathologically, the myocardial fiber in group C showed irregular alignment, disruption, and mitochondrial expansion. Conclusion A sudden increase of altitude to 2260 m in winter can potentially cause hypoxic cardiomyocyte damage as a result of oxidative and environmental stresses.