交通医学
交通醫學
교통의학
MEDICAL JOURNAL OF COMMUNICATIONS
2014年
1期
30-32,35
,共4页
沈卫东%刘鹏飞%刘兵团%许庆华%张伟%项斌
瀋衛東%劉鵬飛%劉兵糰%許慶華%張偉%項斌
침위동%류붕비%류병단%허경화%장위%항빈
胃癌%空泡型质子泵%转化生长因子-β1%免疫组织化学法
胃癌%空泡型質子泵%轉化生長因子-β1%免疫組織化學法
위암%공포형질자빙%전화생장인자-β1%면역조직화학법
gastric cancer%the vacuolar H+-adenosine triphosphatase%transfer growth factor-β1%immunohisto-chemistry
目的:探讨空泡型质子泵(V-ATPases)与转化生长因子-β1(TGF-β1)在胃癌组织中的表达,并探讨其临床意义。方法:运用免疫组化方法检测胃癌组织及癌旁组织中V-ATPases与TGF-β1的表达水平,并分析两者相关性及与胃癌临床病理参数之间的关系。结果:免疫组化结果显示,(1)V-ATPases表达胃癌组织45例中阳性34例(75.5%),癌旁组织阳性13例(28.8%),两者比较差异有统计学意义(P<0.05)。(2)TGF-β1表达胃癌组织阳性35例(77.8%),癌旁组织阳性15例(33.3%),两者比较差异有统计学意义(P<0.05)。(3)相关性分析结果:V-ATPases的表达与TGF-β1的表达呈正相关(P<0.05);V-ATPases与TGF-β1的表达与病理分级、淋巴结转移、TNM分期、脉管浸润呈正相关(P<0.05),与临床病理特征如性别、年龄、肿瘤大小无相关性。结论:在胃癌组织中高表达的V-ATPases与TGF-β1可能胃癌发生浸润转移的重要生物学标志,检测V-ATPases与TGF-β1对判断胃癌浸润转移具有重要意义。
目的:探討空泡型質子泵(V-ATPases)與轉化生長因子-β1(TGF-β1)在胃癌組織中的錶達,併探討其臨床意義。方法:運用免疫組化方法檢測胃癌組織及癌徬組織中V-ATPases與TGF-β1的錶達水平,併分析兩者相關性及與胃癌臨床病理參數之間的關繫。結果:免疫組化結果顯示,(1)V-ATPases錶達胃癌組織45例中暘性34例(75.5%),癌徬組織暘性13例(28.8%),兩者比較差異有統計學意義(P<0.05)。(2)TGF-β1錶達胃癌組織暘性35例(77.8%),癌徬組織暘性15例(33.3%),兩者比較差異有統計學意義(P<0.05)。(3)相關性分析結果:V-ATPases的錶達與TGF-β1的錶達呈正相關(P<0.05);V-ATPases與TGF-β1的錶達與病理分級、淋巴結轉移、TNM分期、脈管浸潤呈正相關(P<0.05),與臨床病理特徵如性彆、年齡、腫瘤大小無相關性。結論:在胃癌組織中高錶達的V-ATPases與TGF-β1可能胃癌髮生浸潤轉移的重要生物學標誌,檢測V-ATPases與TGF-β1對判斷胃癌浸潤轉移具有重要意義。
목적:탐토공포형질자빙(V-ATPases)여전화생장인자-β1(TGF-β1)재위암조직중적표체,병탐토기림상의의。방법:운용면역조화방법검측위암조직급암방조직중V-ATPases여TGF-β1적표체수평,병분석량자상관성급여위암림상병리삼수지간적관계。결과:면역조화결과현시,(1)V-ATPases표체위암조직45례중양성34례(75.5%),암방조직양성13례(28.8%),량자비교차이유통계학의의(P<0.05)。(2)TGF-β1표체위암조직양성35례(77.8%),암방조직양성15례(33.3%),량자비교차이유통계학의의(P<0.05)。(3)상관성분석결과:V-ATPases적표체여TGF-β1적표체정정상관(P<0.05);V-ATPases여TGF-β1적표체여병리분급、림파결전이、TNM분기、맥관침윤정정상관(P<0.05),여림상병리특정여성별、년령、종류대소무상관성。결론:재위암조직중고표체적V-ATPases여TGF-β1가능위암발생침윤전이적중요생물학표지,검측V-ATPases여TGF-β1대판단위암침윤전이구유중요의의。
Objective: To investigate the expression of V-ATPases and TGF-β1 in gastric cancer and their clinical significance. Methods: Immunohistochemistry and western blot were used to detect the expression of V-ATPases and TGF-β1 in gastric cancer specimens, respectively. The correlations among the V-ATPases, TGF-β1 protein, and clinico-pathological parameters of gastric cancer were analyzed. Results: In the assay of immunohistochemistry showed that the positive expression rates of V-ATPases were 75.5%and 28.8%in gastric cancer and the adjacent tissue, respectively. The positive expression rates of TGF-β1 were 77.8 % and 33.3 % in gastric cancer and the adjacent tissue, respectively. Sig-nificant differences (P<0.01) were found between the two aforementioned groups. The correlation analysis indicated that the expression of V-ATPases had a close positive correlation with that of TGF-β1 in gastric cancer (P<0.01). The up-regula-tion of V-ATPases expression was associated with lymph node metastasis, TNM, vascular invasion and differentiation (P<0.01). The expression of the TGF-β1 expression was associated with an advanced clinical stage, infiltrating depth, lymph node metastasis and vascular invasion in the cancer (P<0.01). Conclusion:The high expression of V-ATPases and t TGF-β1 protein may be important biological markers for malignant transformation in the invasion and metastasis of gastric can-cer. The detection of V-ATPases and TGF-β1 expression in gastric cancer is significantly important for improving the pre-diction of invasion and metastasis.