CAJ | 학술논문

针对两种典型的有害浮游植物微小原甲藻和Takayama pulchellum（T．pulchellum）各设计了4条特异性探针，并采用基于PCR管载体的荧光原位杂交检测方法检测了这些探针的标记效果。实验结果表明，针对微小原甲藻和T．pulchellum核糖体大、小亚基DNA（LSU和SSU rDNA）的序列信息所设计的8条荧光标记探针均能够特异性地识别这些目标藻。各探针的标记效果有一定差异，经过标记的目标藻细胞在单种和自然水样混合样品中均可以通过荧光显微镜进行识别和区分。针对微小原甲藻和T．pulchellum的荧光原位杂交检测方法的建立将有助于对样品中这些目标藻进行快速准确地检测和监测。
침대량충전형적유해부유식물미소원갑조화Takayama pulchellum（T．pulchellum）각설계료4조특이성탐침，병채용기우PCR관재체적형광원위잡교검측방법검측료저사탐침적표기효과。실험결과표명，침대미소원갑조화T．pulchellum핵당체대、소아기DNA（LSU화SSU rDNA）적서렬신식소설계적8조형광표기탐침균능구특이성지식별저사목표조。각탐침적표기효과유일정차이，경과표기적목표조세포재단충화자연수양혼합양품중균가이통과형광현미경진행식별화구분。침대미소원갑조화T．pulchellum적형광원위잡교검측방법적건립장유조우대양품중저사목표조진행쾌속준학지검측화감측。
Four specific DNA probes were designed to identify Prorocentrum minimum and Takayama pulchellum two harmful phytoplankton, and fluorescence in situ hybridization probes based on PCR tube carriers were developed .Epifluorescence mi-crographs showed that these specific probes labeled with fluorescein isothiocyanate can identified target sequences , and the flu-orescence signal resulted from whole -cell hybridization presented differences .The hybridization cell can be identified in pured and mixed natural algal samples .The results suggested that method of P.minimum and T.pulchellum fluorescence in situ hybridization based DNA probes could achieve fast and accurately detection of target algaes .