CAJ | 학술논문

以钦州湾小球藻为原料，通过单因素和正交试验探讨酶解法制备多肽的最佳工艺。实验结果表明：酶解小球藻蛋白制备多肽的最佳工艺条件为：pH 6.5，酶解温度30℃，酶解时间20 min，料液比1∶100 g/mL。此时多肽得率为15.538%±0.2%。
이흠주만소구조위원료，통과단인소화정교시험탐토매해법제비다태적최가공예。실험결과표명：매해소구조단백제비다태적최가공예조건위：pH 6.5，매해온도30℃，매해시간20 min，료액비1∶100 g/mL。차시다태득솔위15.538%±0.2%。
In this study, using single factor and orthogonal experiments, the prepare process optimization of polypeptides from chlorella protein of Qinzhou Bay were investigated by enzyme hydrolysis. The results showed that optimum conditions for prepare polypeptides were as follows:pH is 6.5, the hydrolysis temperature and time is 30℃and 20 min, chlorella was mixed with phosphate buffer at ratio of 1∶100 g/mL. At the condition, yield of polypeptides is 15.538%±0.2%.