CAJ | 학술논문

利用高效液相色谱（HPLC）技术对24种1变种柃属植物28份样品的化学成分进行检测，并以各样品中色谱峰的相对保留时间对柃属植物的特征化学成分进行标记，最终统计到23个特征色谱峰用于化学分类研究。结果显示，柃属植物色谱峰在种内稳定出现，在种间则呈现多样性，可用于物种的鉴别。为进一步探讨种间亲缘关系及部分种的具体分类学地位，研究同时还采用UPGMA法和NJ法对色谱峰进行聚类分析，并根据两种聚类树型图讨论了化学成分的分类学意义。研究结果表明HPLC色谱技术具有快捷性、直观性和重现好的特点，可作为一种理想的标记用于柃属植物的分类鉴定。此外，本研究还能为柃属进一步的资源利用研究提供重要的参考。
이용고효액상색보（HPLC）기술대24충1변충령속식물28빈양품적화학성분진행검측，병이각양품중색보봉적상대보류시간대령속식물적특정화학성분진행표기，최종통계도23개특정색보봉용우화학분류연구。결과현시，령속식물색보봉재충내은정출현，재충간칙정현다양성，가용우물충적감별。위진일보탐토충간친연관계급부분충적구체분류학지위，연구동시환채용UPGMA법화NJ법대색보봉진행취류분석，병근거량충취류수형도토론료화학성분적분류학의의。연구결과표명HPLC색보기술구유쾌첩성、직관성화중현호적특점，가작위일충이상적표기용우령속식물적분류감정。차외，본연구환능위령속진일보적자원이용연구제공중요적삼고。
Chemotaxonomic study of 25 taxa (24 species and 1 variety)of Eurya,in a total of 28 sam-ples were carried with high performance liquid chromatograms method.Twenty-three chemical compo-nents from the whole samples were recognized according to the relative retention time of the chromato-grams.The results showed that the chromatograms stabilized between same species and varied among dif-ferent species,which could be used to distinguish species from each other.In order to reveal the inter-specific relationships and the taxonomic positions of these species,chromatograms data were submitted to cluster analysis using UPGMA and NJ methods and then the taxonomic significance of chemical compo-nent in genus Eurya were discussed based on the two dendrograms.Our study showed that the HPLC chromatograms method was rapid,intuitive and repeatable,and it could be mentioned as a good marker to classify the plants of Eurya.In addition,this study also provided useful data for studying the resource utilization in the genus.