CAJ | 학술논문

目的：观察芪龙头痛颗粒的镇痛作用,探讨其治疗偏头痛的作用机制。方法昆明种小鼠随机分为空白组、正天丸组、布洛芬组及芪龙头痛颗粒高、中、低剂量组。灌胃3d后,采用热板法和醋酸扭体法评估芪龙头痛颗粒的镇痛作用。SD 大鼠随机分为空白组、正天丸组、模型组、曲普坦组及芪龙头痛颗粒高、中、低剂量组。连续灌胃7 d后,腹腔注射硝酸甘油(10 mg/kg),4 h后,下腔静脉采血检测血浆降钙素基因相关肽(CGRP)和β-内啡肽(β-EP)水平。然后经心脏灌流,获取含有三叉神经脊束核尾段(Sp5C)的脑组织,采用免疫组化方法检测Fos蛋白表达。结果芪龙头痛颗粒可显著提高小鼠的痛阈值,减少醋酸扭体反应次数；显著下调模型大鼠血浆CGRP水平,上调血浆β-EP水平,抑制Sp5C的Fos蛋白表达。结论芪龙头痛颗粒具有良好的镇痛作用,其治疗偏头痛的机制可能是通过调节神经递质或神经肽水平及抑制Fos通路的活化发挥作用。
목적：관찰기룡두통과립적진통작용,탐토기치료편두통적작용궤제。방법곤명충소서수궤분위공백조、정천환조、포락분조급기룡두통과립고、중、저제량조。관위3d후,채용열판법화작산뉴체법평고기룡두통과립적진통작용。SD 대서수궤분위공백조、정천환조、모형조、곡보탄조급기룡두통과립고、중、저제량조。련속관위7 d후,복강주사초산감유(10 mg/kg),4 h후,하강정맥채혈검측혈장강개소기인상관태(CGRP)화β-내배태(β-EP)수평。연후경심장관류,획취함유삼차신경척속핵미단(Sp5C)적뇌조직,채용면역조화방법검측Fos단백표체。결과기룡두통과립가현저제고소서적통역치,감소작산뉴체반응차수；현저하조모형대서혈장CGRP수평,상조혈장β-EP수평,억제Sp5C적Fos단백표체。결론기룡두통과립구유량호적진통작용,기치료편두통적궤제가능시통과조절신경체질혹신경태수평급억제Fos통로적활화발휘작용。
Objective To observe the analgesic effect of Qilong Toutong Granule and explore its underlying mechanisms of action. Methods Kunming mice were randomly divided into blank group, Zhengtian pill group, ibuprofen group, and high-, medium- and low-dose of Qilong Toutong Granule groups. After completing the prophylactic treatment for 3 days, hot-plate test and acetic acid-induced writhing test were used to observe the pain reaction of mice. SD rats were randomly divided into blank group, Zhengtian pill group, model group, rizatriptam group, and high-, medium- and low-dose of Qilong Toutong Granule groups. After gavaged for continuous 7 days, rats were intraperitoneally injected nitroglycerin (10 mg/kg), and 4 hours later, blood samples were collected from postcava for measuring the levels of plasma CGRP and β-EP by radioimmunoassay. Subsequently, rats were perfused transcardially and the brain tissues containing the trigeminal nucleus caudalis (Sp5C) were achieved for detecting the expression of Fos protein by immunohistochemical method. Results Qilong Toutong Granule significantly raised the pain threshold, reduced the number of writhing response of mice, remarkably down-regulated the levels of plasma CGRP, up-regulated the levels of plasmaβ-EP and inhibited the expression of Fos protein in Sp5C of rats compared with the model group. Conclusion Qilong Toutong Granule has good analgesic effect and its mechanisms on relieving migraine may be through regulating the levels of neurotransmitters or neuropeptides, and inhibiting the activation of Fos pathway.