中国中医药信息杂志
中國中醫藥信息雜誌
중국중의약신식잡지
CHINESE JOURNAL OF INFORMATION ON TRADITIONAL CHINESE MEDICINE
2014年
2期
53-55
,共3页
镰形棘豆%生物碱%致病菌%抑菌作用
鐮形棘豆%生物堿%緻病菌%抑菌作用
렴형극두%생물감%치병균%억균작용
Oxytropis falcata Bunge%alkaloids extract%pathogens%antibacterial activity
目的:观察镰形棘豆生物碱提取物对9种常见致病菌的抗菌作用。方法以镰形棘豆为原料,提取生物碱,选择临床常见的4种革兰氏阳性菌和5种革兰氏阴性菌,采用琼脂扩散法对其体外抑菌活性进行评价,试管二倍稀释法测定其最低抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果镰形棘豆生物碱提取物对9个菌种均有抑菌效果,抑菌作用敏感度与浓度呈良好的量效关系,其中对枯草杆菌和嗜麦芽窄食单胞菌的抑菌效果优于其他各菌,MIC和MBC分别为0.95 mg/mL和1.91 mg/mL,而其他各菌种的MIC和MBC范围在1.91~7.63 mg/mL之间。结论镰形棘豆生物碱提取物具有较为广谱的抑菌作用,对革兰氏阳性菌的抑制作用比革兰氏阴性菌强。
目的:觀察鐮形棘豆生物堿提取物對9種常見緻病菌的抗菌作用。方法以鐮形棘豆為原料,提取生物堿,選擇臨床常見的4種革蘭氏暘性菌和5種革蘭氏陰性菌,採用瓊脂擴散法對其體外抑菌活性進行評價,試管二倍稀釋法測定其最低抑菌濃度(MIC)和最小殺菌濃度(MBC)。結果鐮形棘豆生物堿提取物對9箇菌種均有抑菌效果,抑菌作用敏感度與濃度呈良好的量效關繫,其中對枯草桿菌和嗜麥芽窄食單胞菌的抑菌效果優于其他各菌,MIC和MBC分彆為0.95 mg/mL和1.91 mg/mL,而其他各菌種的MIC和MBC範圍在1.91~7.63 mg/mL之間。結論鐮形棘豆生物堿提取物具有較為廣譜的抑菌作用,對革蘭氏暘性菌的抑製作用比革蘭氏陰性菌彊。
목적:관찰렴형극두생물감제취물대9충상견치병균적항균작용。방법이렴형극두위원료,제취생물감,선택림상상견적4충혁란씨양성균화5충혁란씨음성균,채용경지확산법대기체외억균활성진행평개,시관이배희석법측정기최저억균농도(MIC)화최소살균농도(MBC)。결과렴형극두생물감제취물대9개균충균유억균효과,억균작용민감도여농도정량호적량효관계,기중대고초간균화기맥아착식단포균적억균효과우우기타각균,MIC화MBC분별위0.95 mg/mL화1.91 mg/mL,이기타각균충적MIC화MBC범위재1.91~7.63 mg/mL지간。결론렴형극두생물감제취물구유교위엄보적억균작용,대혁란씨양성균적억제작용비혁란씨음성균강。
Objective To investigate the antibacterial activity of the alkaloids extract from Oxytropis falcata Bunge against 9 common pathogens in vitro. Methods Alkaloids was extracted from Oxytropis falcata Bunge. The agar plate dilution method was adopted to evaluate the activity against bacteria of 4 Gram-negative test strains and 5 Gram-positive test strains, while the MIC and MBC were determined by tube double dilution method. Results The alkaloids extract from Oxytropis falcata Bunge had the antibacterial activity against 9 pathogens and there was a good dose-response relationship between sensitivity and concentration. Antibacterial activities against bacillus subtilis and stentrophmomas maltophilia showed stronger than other tested bacteria, the MIC and MBC were 0.95 mg/mL and 1.91 mg/mL, respectively. In other tested bacteria, MIC and MBC were ranged in 1.91-7.63 mg/mL. Conclusion The alkaloids extract showed a broad spectrum of a stronger antibacterial activity against all tested pathogens. Gram-positive strains were more sensitive than Gram-negative strains to the inhibitory effects of the alkaloids extract.